沙利度胺联合吉西他滨对裸鼠胰腺癌细胞株(sw-1990)皮下移植瘤生长的影响-effect of thalidomide combined with gemcitabine on the growth of subcutaneous transplanted tumor of pancreatic cancer cell line ( sw - 1990 ) in nude mice.docxVIP

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沙利度胺联合吉西他滨对裸鼠胰腺癌细胞株(sw-1990)皮下移植瘤生长的影响-effect of thalidomide combined with gemcitabine on the growth of subcutaneous transplanted tumor of pancreatic cancer cell line ( sw - 1990 ) in nude mice.docx

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沙利度胺联合吉西他滨对裸鼠胰腺癌细胞株(sw-1990)皮下移植瘤生长的影响-effect of thalidomide combined with gemcitabine on the growth of subcutaneous transplanted tumor of pancreatic cancer cell line ( sw - 1990 ) in nude mice

沙利度胺联合吉西他滨对裸鼠胰腺癌细胞株（SW-1990）皮下移植瘤生长的影响英文摘要Effectofthalidomidecombinedwithgemcitabineonthegrowthofhumanpancreaticcarcinoma(SW-1990)xenograftinnudemiceAbstractObjectiveTostudytheinhibitoryeffectsofthalidomide（THD）combinedwithgemcitabine（GEM）onthegrowthofhumanpancreaticcarcinoma（SW1990）innudemicesubcutaneousxenograftmodel.MethodsThecelllineSW1990inlogphaseofhumanpancreaticcarcinomawasimplantedsubcutaneouslyinBALB/Cnudemicetodevelopimplant-tumor.The20nudemicewererandomlyallocatedintofourgroups:groupA:injectsalineIP;groupB:injectTHD200mg/kg·diptiw;groupC:injectGEM50mg/kg·dipbiw;groupD:injectTHD200mg/kg·dIPtiwandinjectGEM50mg/kg·dIPbiw.AftertreatingwithTHDor(and)GEM,thetumorgrowthinmicewereobserved.After28days,themicewerekilledandthetumorswereimmediatelyremovedforfurtherstudies.Thevolumeandweightofimplant-tumorweremeasuredandtheratesofcancerinhibitionwereevaluated.TheexpressionofVEGF,Bcl-2andBaxweredetectedbyRT-PCR;SimultaneouslythemethodofimmunohistochemistryandwesternblotwereusedtoestimatetheexpressionofVEGFandcaspase-3;Microvesseldensity(MVD)wascountedbyendothelialcellsimmunostainedbyanti-CD34antibody.ResultsNoneofthenudemicediedandallofthemformedimplant-tumor.1.thesizeoftumorofcontrolgroup,THDgroup,GEMgroupandcombinedgroupwere(1.256±0.128)cm3、(0.898±0.142)cm3、(0.720±0.124)cm3、(0.442±0.117)cm3,andtheweightswere(1.412±0.138)g、(1.013±0.955)g、(0.864±0.112)g、(0.512±0.910)g.Comparedwiththecontrolgroup,growthoftumorwassignificantlyreducedinmicetreatedwiththalidomide,gemcitabineandcombinedtreatment(P0.05),especiallythecombinedgroup.Theinhibitionrateofthalidomidegroup,gemcitabinegroupandcombinedgroupwere(28.3±3.2)%、(38.8±4.2)%、(63.7±6.0)%.2.Comparedwiththecontrolgroup,thelevelsofVEGFmRNAandBcl-2mRNAweredownregulatedinTHDgroup,GEMgroup英文摘要沙利度胺联合吉西他滨对裸鼠胰腺癌细胞株（SW-1990）皮下移植瘤生长的影响andcombinedgroup,whiletheexpressionofBaxmRNAwasupregulated.ComparedwithTHDorGEMgroups,VEGFmRNA,Bcl-2mRNAorBax-mRNAincombinedgrouphavesignificantdifference(P0.05).3.Comparedwiththecontrolgroup,thelevelofVEG