水稻耐低磷选择导入系产量性状改良与qtl定位分析-improvement of yield traits and qtl mapping analysis of low phosphorus tolerance selection introgression lines in rice.docxVIP

量起作用，加性效应均为负，有利等位基因来源于蜀恢527，在MAS育种中选择表现受体基因个体，可有效地使结实率、千粒重、单株产量得到同步改良。有的一因多效QTL主要产量性状同步改良不利，应根据育种目标，灵活利用，如：在蜀恢527/爷驼崽群体中标记RM169连锁位点同时作用于株高、穗数、穗长、每穗总粒数、结实率和千粒重，其中控制穗数和结实率的有利等位基因来供体，其他性状有利等位基因来自轮回亲本，说明在该位点导入供体基因可增加穗数，提高结实率，但每穗总粒数会降低。关键词：水稻，耐低磷，选择导入系，产量性状，数量性状位点（QTL）IIAbstractThelowutilizationrateofphosphorusisamajorlimitingfactorinriceproduction.Inordertoexplorethefavorablegenesofricetolerancetolowphosphorus,phosphorusfosterefficientvarieties,inourstudy,fourBC2F4backcrossingpopulationsweredevelopedfromtheeliteindicarestorerlinesShuhui527andMinghui86astherecurrentparents,YetuozaiandPokhreliasdonorparents.Yieldanditsrelatedtraitsofthebackcrossingpopulationswereevaluatedandanalyzedundernormalcontrolandphosphorusdeficiencyconditions.SSRpolymorphicmolecularmarkerswereusedforgenotypicanalysis.One-wayanalysisofvariances(ANOVA)andchi-squaretestwereappliedtoidentifyQTLsforallthemeasuredtraits.Theresultsofthestudysuggestedthatthevariationofthousandgrainweight(TGW)andpaniclelength(PL)issmall.Therewasagreatervariationoftraitswhichwereconstrainedbytheenvironmentfactor,suchaspaniclenumber(PN),?lled-grainnumberperpanicle(FGP)andspikeletfertility(SF).LowphosphorusstresshaveagreaterimpactonPN,FGPandYD,ofwhich,PNwasreducedamaximumdegreebylowphosphorusstress,leadingtoaloweryield.TheresultsofcorrelationanalysisshowedthatYDandPN,FGP,spikeletsperpanicle(TNSP)wereinahighlysignificantorsignificantpositivecorrelationundernormalandlowphosphorusstressconditions.ThecorrelationbetweenPNandFGPismanifestedasasignificantorverysignificantnegativelevel.Thus,weshouldnotonlypayattentiontotheimprovingamountoftillers,butalsotakefilled-grainnumberintoaccountinthepracticebreeding.Theresultsofpathanalysisshowedthat,PNprovidedthelargestdirecteffectforYDundernormalconditionsinfourpopulations.Thisresultisconsistentwiththecorrelationcoefficientanalysisresults.FGPprovidedthelargestdirecteffectforYDinthetwopopulationsofMinghui86backgroundunderthelowphosphorusconditions,indicatingthatFGPshouldbeconsideredinthisgene