Gram Negative Rods UNMC Home挑剔的革兰阴性杆菌内布拉斯加大学医学中心的家庭ppt课件.pptVIP

Fastidious Gram Negative RodsBlood Culture Unit;Fastidious Complex / extensive nutrient requirements Faint staining Gram Negative Rods Safranin counterstain for 2 minutes Substitute carbolfuschin for safranin Serological testing useful;Brucella speciesClinical Significance;Blood, bone marrow or tissue Risk of lab acquired infection Specify on requisition when suspecting Brucella BAP, CHOC, BCYE, Blood Culture broth;35C, 5-10% CO2 for 21 days Colony morphology Growth at 7 days BAP = smooth glistening, translucent colonies that become brown with age;Gram stain: tiny, faint staining gram