石菖蒲的抗焦虑作用分析-analysis of anxiolytic effect of acorus tatarinowii schott.docxVIP

摘要目的：观察石菖蒲抗焦虑作用并探讨其作用机制。方法：石菖蒲的抗焦虑实验：小鼠随机分为模型对照组、地西泮（DZP）组、挥发油高、中、低剂量组,水提液高、中、低剂量组,共8组，每组12只,每组均连续灌胃5天后，在小鼠高架十字迷宫箱装置、明暗箱装置上观察石菖蒲对小鼠焦虑行为的影响，并采用酶联免疫法测定小鼠脑内r-氨基丁酸（GABA）、谷氨酸（Glu）和5-羟色胺（5-HT）递质含量。结果：①在小鼠高架十字迷宫模型中，石菖蒲挥发油高剂量组和石菖蒲水煎液中、低剂量组对小鼠有显著的的抗焦虑作用。②在小鼠明暗箱模型中，石菖蒲挥发油和石菖蒲水煎液各剂量组对小鼠均无显著的的抗焦虑作用，但石菖蒲挥发油中、低剂量组和石菖蒲水煎液的低剂量组表现出抗焦虑作用的趋势。③在石菖蒲对神经递质作用的机理研究中，石菖蒲挥发油各剂量组和石菖蒲水煎液高剂量组能显著提高小鼠脑内的γ-氨基丁酸含量(GABA)（P＜0.05）；石菖蒲挥发油中、低剂量组和石菖蒲水煎液各剂量组能显著降低小鼠脑内的谷氨酸（Glu）含量（P＜0.05）；石菖蒲挥发油各剂量组和石菖蒲水煎液各剂量组能显著降低小鼠脑内的5-羟色胺（5-HT）的含量（P＜0.05），说明石菖蒲的抗焦虑作用，可能与影响GABA、Glu、5-HT的含量有关。结论：①石菖蒲对小鼠高架十字迷宫焦虑模型有一定抗焦虑的作用。②石菖蒲的抗焦虑作用，可能与影响GABA、Glu、5-HT的含量有关。关键词：石菖蒲；高架十字迷宫；明暗箱；抗焦虑；机理StudyonRhizomaAcoriTatarinowiiontheroleofanti-anxietyinmiceZhangZhijuan(TraditionalChineseMateriaMedica)DirectedbyCuiYingAbstractObjective:StudiesontheanxiolyticeffectandfunctionmechanismofRhizomaAcoriTatarinowii.Methods:Theanti-anxietytestofRhizomaAcoriTatarinowiiinmice：Themiceweredividedrandomly,twelvemiceineachgroupintoblankcontrolgroup,diazepamgroup,high-dosegroup,middle-dosegroupandlow-dosegroupofvolatileoilandwaterextract,whichweregivendifferentmedicinefor5daysbeforethebehavioraltests,includingelevatedplusmazetestsandlight-darktests,whichwerecarriedout0.5hafteradministrationtoexaminetheanxietybehaviorofrats.Afterbehavioraltests,thebrainofeachratwasremovedquicklyfromskull.Andwedeterminedr-aminobutyricacid,glutamicacidand5-hydroxytryptamineneurotransmittercontentofmousebraininhoney-Enzyme-linkedimmunosorbentassaymethod.Throughthese,wecanobservedthemechanismsofanti-anxietyeffectsofRhizomaAcoriTatarinowii.Results:①Intheelevatedplusmazetests,high-dosegroupofvolatileoil,middle-dosegroupandlow-dosegroupofwaterextracthaveshownsignificantanxiolyticeffects.②Inthelight-darktests,althoughthemodelissuccessful,theeachgroupofvolatileoilandwaterexracthaveshownnosignificantanxiolyticeffects,butvolatileoilinmiddleandlow-dosegroupandwaterextactinlow-dosegrouphaveshowntrendanxiolyticeffects.③Inthestudyofthemechanismofneurotransmitter,theeachgroupofvolatileoiland