石墨烯修饰玻碳电极用于急性早幼粒细胞白血病pmlrarα融合基因检测的实验分析-experimental analysis of graphene modified glassy carbon electrode for detection of pml rar α fusion gene in acute promyelocytic leukemia.docxVIP

nanotechnologyandpossessessurfaceeffect,smallsizeeffect,quantumsizeeffectandmacroscopicquantumtunnelingeffectwhichmakeitshowspecialcharactersinmechanics,electricity,magnetics,thermotics,optics,chemicalactivity.Carbonmaterialisthehotspotinnanomaterials,suchasfullerene,carbonnanotubeandgrapheneandsoon.Grapheneisthirdlylatestdiscoveredasa‘newpatterncarbonmaterial’.Graphenehastherelativeadvantagessuchaslargespecificsurfacearea,biggeradsorptionfunctionandfasterelectrontransferandelectro-catalyticactivity.Inthepaper,agraphenemodifiedglassycarbonelectrode(GCE)waspreparedbyelectrochemicalreductionofgraphiteoxidefixedonthesurfaceofGCEandcharacterizedusingelectrochemistrymethod.Withmethyleneblue(MB)aselectricalactivitymodelmolecules,theelectro-catalyticfunctionforMBofgraphenemodifiedglassycarbonelectrodewasstudied.Theresultsindicatedthatgraphiteoxidebecamegraphemethroughelectrochemicalreductionandgraphenemodifiedglassycarbonelectrodewasbuiltsuccessfully.ThemodifiedelectrodeshowedgoodstabilityandmaximumsensitizationfortheredoxcurrentofMB.Theelectro-catalyticfunctionoftheelectrodewasfine.Onthatbasis,thegraphenemodifiedglassycarbonelectrodewasusedfordetectionofacutepromyelocyticleukemia(APL)PML/RARαfusiongene.Theamino-groupmodifiedprobeofAPLPML/RARαfusiongenefragmentwasimmobilizedontosurfaceofthegraphenemodifiedGCEbycouplingactivatingagent.WithMBasanovelelectrochemicalindicator,artificialAPLPML/RARαfusiongenefragmentwasmeasuredbydifferentialpulsevoltammetry(DPV).TheresultindicatedgraphenenanomaterialshowedgoodsensitizationonthemeasurementsignalofMB.TherelationshipbetweenthereductionpeakcurrentofMBandtheconcentrationofcomplementarystrandwaslinearintherangeof5×10-10~2.5×10-9mol/L.Thedetectionlimithadbeenestimatedas8×10-11mol/L.Atthesametime,thecharactersofthebiosensor,suchasspecificityandstabilityandsoon,wereinvestigated.Themethodissimple,goodspecificityandhighsensitivity.Thisapproachispromisingtothedetectionoftherealsample.Keywords:Graphene;Graphitoxide;Graphenemodifiedglassycarb