大鼠阴道上皮细胞体外培养研究进展.docVIP

大鼠阴道上皮细胞体外培养研究进展 　　摘要：外阴阴道假丝酵母菌病（VVC）是由假丝酵母菌引起的机会性真菌感染，约5%～10%的VVC患者可因病情反复发作成为复发性外阴阴道假丝酵母菌病（RVVC）患者。目前认为免疫异常是导致RVVC的主要因素，确切机制不明。大鼠阴道上皮细胞的体外培养为该病免疫机制的研究提供更多可能，其培养方法主要有组织块法和酶消化法，一般认为酶消化法优于组织块法。动情前期和动情期体外培养获得的大鼠阴道上皮细胞数比动情后期和动情间期多，但尚未形成一致的结论。细胞培养基成分也日渐完善，本文就大鼠阴道上皮细胞体外培养的研究进展做一综述。 　　关键词：复发性外阴阴道假丝酵母菌病；阴道上皮；大鼠；细胞培养 　　中图分类号：R711.31 文献标识码：A 文章编号：1006-1959（2017）19-0022-03 　　Research Progress of Vaginal Epithelial Cells of Rat Cultured in Vitro 　　LU Wei，QI Wen-jin 　　（Department of Obstetrics and Gynecology，the First Affiliated Hospital of Kunming Medical University，Kunming 650000，Yunnan，China） 　　Abstract：Vulvovaginal candidiasis（VVC）is an opportunistic fungal infection caused by Candida，about 5% to 10% of VVC patients may be recurrent as a result of recurrent vulvovaginal candidiasis（RVVC）patient.At present，the immune abnormalities are the main factors leading to RVVC，the exact mechanism is unknown.In vitro culture of rat vaginal epithelial cells provides more possibilities for the study of the immune mechanism of the disease. The culture method is mainly composed of tissue block method and enzyme digestion method.It is generally believed that the enzymatic digestion method is superior to the tissue block method.The number of vaginal epithelial cells in the prenatal and estrous stage was higher than that in the late stage and the estrous stage，but the conclusion was not yet reached.Cell culture medium components are increasingly perfect.In this paper，we reviewed the progress in the study of vaginal epithelial cells in vitro. 　　Key words：Recurrent vulvovaginal candidiasis；Vaginal epithelium；Rat；Cell culture 　　外阴阴道假丝酵母菌病（vulvovaginal candidiasis，VVC）是由假丝酵母菌感染引起的外阴阴道炎症，在阴道感染中居第2位，仅次于细菌性阴道炎[1]。据资料统计，70%～75%的女性一生中至少患VVC 1次，其中40%～50%患有VVC的女性会有复发感染，5%～10%的女性一年内发作4次或4次以上而成为复发性假丝酵母菌外阴阴道炎（recurrent vulvovaginal candidiasis，RVVC）[2]。目前认为阴道局部免疫功能异常是导致RVVC的主要因素，但是确切发病机制尚不清楚。大鼠阴道上皮细胞的体外培养为该病免疫机制的研究提供更多可能，其培养方法主要有组织块法和酶消化法，国内外许多学者对这两种方法均有研究，一般认为酶消化法优于组织块法[3-4]，动情前期和动情期体外培养获得的大鼠阴道上皮