石榴皮对大肠湿热证模型小鼠il-1β il-6及siga等影响的实验研究-experimental study on the effect of pomegranate peel on il - 1β il - 6 and siga in large intestine damp-heat syndrome model mice.docxVIP

摘要石榴皮对大肠湿热证模型小鼠IL-1β、IL-6及sIgA等影响的实验研究研究生：冯贵仁导师：单丽娟教授摘要目的：探讨石榴皮对大肠湿热证模型小鼠肠道炎症反应及免疫功能的影响。方法：以清洁级昆明小鼠40只，随机分组。应用高糖高脂饲料、高温高湿环境、产毒性大肠杆菌灌胃，综合制作大肠湿热证动物模型，给予石榴皮煎液反佐验证治疗，用放射免疫法检测鼠血清IL-1β、IL-6及sIgA含量，HE染色法观察结肠、回肠病理改变。结果：大肠湿热证模型鼠出现发热、食欲不振、肛门红肿充血、便溏等表现，近似于中医大肠湿热证型。模型鼠血清IL-1β、IL-6及sIgA含量比正常对照组升高（P0.05），结肠、回肠组织HE染色见粘膜充血、水肿、渗出、淋巴组织增生、大量炎症细胞浸润。石榴皮水煎液治疗鼠症状消失，血清IL-1β、IL-6及sIgA含量与模型组相比（P0.05），光镜显示肠道炎症反应明显减轻。结论：（1）在高糖高脂饲料喂养、大肠杆菌灌胃的基础上，采用全封闭式高温仓，自动调节温度、湿度，所制模型近似大肠湿热证，体现了中医大肠湿热证脾胃内伤，再感客邪的“内外合邪”的致病特点。（2）运用石榴皮对模型大鼠进行反佐验证治疗后大肠湿热证症状消失，血清IL-6、IL-1β及sIgA含量恢复正常，光镜观察肠道炎症改变明显减轻甚至消失，也肯定了疗效。关键词：大肠湿热；疾病模型；细胞因子；石榴皮新疆医科大学医学硕士学位论文StudytheeffectsofIL-1β、IL-6andsIgAonthemicemodeloftraditionalmedicallargeintestinedamp-heatsyndrometreadedwithGranatumName:AmosSupervisor:ShanLijuanABSTRACTObjective:TostudytheeffectsofGranatumonthemicelargeintestinedamp-heatsyndromemodelinthesideofinflammationandimmuneofintestinaltract.Methods:Bymeansofusing40clearinggradeKunmingmousewererandomlydividedintofourgroups.thenanimalmodelwerecreatedwithacomprehensivemethodoffoddersrichofsugarandfatcircumstanceofhighwetandtemperature,pouringescherichiacoliintostomach,Granatumsoupwereusedtotreatwetheatsyndromemodelmouse.ThecontentsofIL-1β、IL-6andsIgAinbloodserumweredetectedwithradioimmunity.HEstainingwasusedtoobservelargeintestine’spathologicalchanges.Results:AllthemanifestationofmouseaftercreatedareasimilarwiththeChinesemedicallargeintestinewetheatsyndrome.TheircontentsofserumIL-1β、IL-6andsIgAwerenotablyhigherthanthoseofwetheatsyndromemodelgrouprats(P0.05).Mucousmembranehyperemia,edema,bleeding,lymphoproliferationandinflammatorycellinfiltrationwereseenbyHEstaining.Lightmicroscopemanifestedthatbowelinflammatoryreactionrelieved.Conclusion:(1)Fodderrichofsugarandfat,pouringrotavirusandcolibacillusintostomachtomakethelargeintestinewetheatsyndrommodeladoptingtotally-enclosedtypehypertherniagranaryfirsttime,whichcanself-adjustmenttemperatureanddegreeofwetness.(2)Itcertificatesthemodelwaslargeintestinewetheatsyndromemodelthroughthesymptom