混合型人乳头瘤病毒的检测ppt课件.ppt

生活家饮食保健孕期选择食用油的学问邢台市第四病院罕见护理应急预案猪气喘病综合防制技术动物营养系列理想蛋白与氨基酸模式的研究进展皮肤病的诊断包括病史体格检查和必要的实验室检查我国有关食物添加剂营养强化剂食物新资本的治理律例与标准 生活家饮食保健孕期选择食用油的学问邢台市第四病院罕见护理应急预案猪气喘病综合防制技术动物营养系列理想蛋白与氨基酸模式的研究进展皮肤病的诊断包括病史体格检查和必要的实验室检查我国有关食物添加剂营养强化剂食物新资本的治理律例与标准 The Hybrid Capture 2 (hc2) SystemHPV DNA TestbyDigeneby Brenda Palacios Objectives State the 2 main low-risk HPV DNA types State the 2 main high-risk HPV DNA types State what type of test method is used for the detection of HPV State the minimum sample volume required for testing State what the molecular sandwich consist of Human Papillomavirus (HPV) Primary etiological agent in cervical cancer 2nd most common type of cancer in women world wide 3rd leading cause of cancer-related deaths in women worldwide Most common viral sexually transmitted infection, that goes undiagnosed due to no symptoms developed HPV Characteristics Non-enveloped double-stranded DNA virus Epitheliotrophic- has great affinity for epithelial cells Obligatory intracellular parasites that deliver their genome and accessory proteins into host cells for viral replication HPV Infection E6 oncogene binds to p53 protein in host cell p53 protein is a negative regulator E6 protein mutates p53 protein removing its protective function Mutation disables p53 gene switch, permitting cell to multiply uncontrolled HPV Types Types 6 11–most common low-risk HPV Associated with genital warts Rarely found in cervical cancer Types 16 18- most common high-risk HPV Associated with cancers of the cervix, vagina, vulva, anus, and penis HPV Detection Traditional screening by Papanicolau (pap smear) test Used universally for initial detection of intraepithelial abnormalities In case of atypical squamous cells of undetermined significance HPV DNA detection is recommended .au/.../sep/cells_image_inside.jpg Molecular Testing Hybrid Capture 2 (hc2) System HPV DNA Test Approved by FDA Nucleic acid hybridization assay No target DNA amplification Single amplification usin