实验五1酶联免疫吸附试验（ELISA）2补体结合反应课件.pptVIP

实验五 1.酶联免疫吸附试验（ELISA)2.补体结合反应;1.酶联免疫吸附试验（ELISA);免疫标记技术;免疫酶测定法(Enzyme ImmunoAssay,EIA);酶联免疫吸附试验(Enzyme-Linked ImmunoSorbent Assay,ELISA);酶联免疫吸附试验(Enzyme-Linked ImmunoSorbent Assay,ELISA);实验内容： 间接法测定溶血素 ——定性检测;实验材料;实验方法;取出包被好抗原的酶标板（4孔/组），甩干孔内液体;预期结果;注意事项;2.补体结合反应（Complement fixation Test, CFT）;概念：;补反中包括两个系统五种成分： 待检系统：已知Ag或Ab、待检Ab或Ag及仅供一组Ag-Ab系统反应的定量补体。 指示系统：绵羊红细胞和溶血素。;待测系统 抗原+未知血清;Ag;抗原：伤寒菌裂解产物 待测血清：56℃，30min 补体：豚鼠血清 2%绵羊红细胞 溶血素;方法：;预期结果：;绵羊红细胞用前应轻晃混匀，不可剧烈震荡。 各种试剂的吸管不可混用。;0C4F7IaMdPgSkVnZq$t*x-A1D5G8JbNeQiTlWo#r%u(y+B3E6H9LcOfRjUmYp!sw)z0C4F7JaMdPhSkVnZq$u*x-A2D5G8KbNfQiTlXo#r%v(y0B3E6I9LcOgRjVmYp!tw)z1C4F7JaMePhSkWnZq$u*x+A2D5H8KbNfQiUlXo#s%v(y0B3F6I9LdOgRjVmYq!tw-z1C4G7JbMePhTkWnZr$u(x+A2E5H8KcNfQiUlXp#s%v)y0B3F6IaLdOgSjVmYq!t*w-z1D4G7JbMeQhTkWoZr$u(x+B2E5H9KcNfRiUmXp#sv)y0C3F7IaLdPgSjVnYq$t*w-A1D4G8JbMeQhTlWoZr%u(x+B2E6H9KcOfRiUmXp!sv)z0C3F7IaMdPgSkVnYq$t*x-A1D5G8JbNeQiTlWo#r%u(y+B3E6H9LcOfRjUmXp!sw)z0C4F7IaMdPhSkVnZq$t*x-A2D5G8KbNeQiTlXo#r%v(y+B3E6I9LcOgRjUmYp!tw)z1C4F7JaMePhSkWnZq$u*x-A2D5H8KbNfQiTlXo#s%v(y0B3E6I9LdOgRjVmYp!tw-z1C4G7JaMePhTkWnZr$u*x+A2E5H8KcNfQiUlXp#s%v)y0B3F6IaLdOgSjVmYq!tw-z1D4G7JbMePhTkWoZr$u(x+A2E9LdOgRjVmYp!tw-z1C4G7JaMePhTkWnZr$u*x+A2E5H8KcNfQiUlXo#s%v)y0B3F6I9LdOgSjVmYq!tw-z1D4G7JbMePhTkWoZr$u(x+A2E5H9KcNfRiUlXp#sv)y0C3F6IaLdPgSjVnYq!t*w-A1D4G8JbMeQhTkWoZr%u(x+B2E5H9KcOfRiUmXp#sv)z0C3F7IaLdPgSkVnYq$t*w-A1D5G8JbNeQ!tw)z1C4G7JaMePhSkWnZr$u*x+A2D5H8KcNfQiUlXo#s%v)y0B3F6I9LdOgSjVmYq!tw-z1D4G7JbMePhTkWoZr$u(x+A2E5H9KcNfRiUlXp#s%v)y0C3F6IaLdOgSjVnYq!t*w-z1D4G8JbMeQhTkWoZr%u(x+B2E5H9KcOfRiUmXp#sv)z0C3F7IaLdPgSkVnYq$t*w-A1D4G8JbNeQhTlWoZr%u(y+B2E6H9KcOfRjUmXp!sv)z0C4F7IaMdPgSkVnZq$t*x-A1D5G8KbNeQiTlWo#r%v(y+B3E6H9LcOfRjUmYp!sw)z0C4F7JaMdPhSkVnZq$u*x-A2D5G8KbNfQiTlXo#r%v(y0B3E6I9LcOgRjVmYp!tw)z1C4G7JaMePhSkWnZr$u*x+A2D5H8KbNfQiUlXo#s%v(y0B3F6I9LdOgRjVmYq!tw-z1C4G7JfQiTlXo#r%v(y0B3E6I9LcOgRjVmYp!tw)z1C4F7JaMePhSkWnZq$u*x+A2D5H8KbNfQiUlXo#s%v(y0B3F6I9LdOgRjVmYq!tw-z1C4G7JbMePhTkWnZr$u(x+A2E5H8KcNfRiUlXp#s%v)y0B3F6IaLdOgSjVmYq!t*w-z1D4G7JbM