燃料乙醇 乙酰丙酸及其酯类衍生物的制备工艺分析-preparation process analysis of ethanol levulinic acid and its ester derivatives as fuel.docxVIP

generation. The high glucose concentration is not conducive to levulinic acid generated, and the higher concentration of substrate, the lower yield of levulinic acid; the yield of levulinic acid increased from 44.3 mol% to 65.9 mol% by feed batch method on high concentration of glucose(18 wt%). Enzyme-catalyzed esterification for levulinate esters synthesis and kinetic analysis: The synthesis of levulinate esters was successfully catalyzed by lipase through esterification reaction in a nonaqueous system. Under optimized reaction conditions (Novozym435, MTBE, 50oC, 0.66U·mL-1 of enzyme loading, levulinic acid/ethanol=1:5), 96.5% of conversion was obtained and the immobilized lipase can be reused at least 4 cycles with half decline of activity. A kinetic model was established based on the classical Ping-Pong Bi-Bi mechanism as well as the experimental data. The kinetic parameters were obtained through fitting the kinetic model with the NS fit module in Origin 8.5: Vmax=0.290 mol·L-1·h-1，KA=0.381 mol·L-1，KB=2.340 mol·L-1，KiB=2.356 mol·L-1. KEY WORDS: acid hydrolysis, kinetic model, bioethanol, pretreatment, levulinic acid, levulinate ester, lipase iii 目 录 中文摘要 i ABSTRACT ii HYPERLINK \l _bookmark0 前 言1 HYPERLINK \l _bookmark1 第一章 文献综述2 HYPERLINK \l _bookmark2 1.1 木质纤维素稀酸水解研究2 HYPERLINK \l _bookmark3 1.1.1 木质纤维素结构与性质2 HYPERLINK \l _bookmark4 1.1.2 反应机理与动力学模型4 HYPERLINK \l _bookmark5 1.2 纤维素燃料乙醇生产工艺6 HYPERLINK \l _bookmark6 1.2.1 研究意义6 HYPERLINK \l _bookmark7 1.2.2 原料预处理7 HYPERLINK \l _bookmark8 1.2.3 纤维素糖化发酵产醇8 HYPERLINK \l _bookmark9 1.2.4 酶回收利用9 HYPERLINK \l _bookmark10 1.3 平台化学品—乙酰丙酸10 HYPERLINK \l _bookmark11 1.3.1 基本化学特性10 HYPERLINK \l _bookmark12 1.3.2 生产方法 11 HYPERLINK \l _bookmark13 1.3.3 研究现状13 HYPERLINK \l _bookmark14 1.4 乙酰丙酸酯类化合物14 HYPERLINK \l _bookmark15 1.4.1 工业应用14 HYPERLINK \l _bookmark16 1.4.2 制备方法15 HYPERLINK \l _bookmark17 1.5 本文研究内容17 HYPERLINK \l _bookmark18 第二章 酸水解法分离玉米芯中五碳糖和六碳糖动力学研究19 HYPERLINK \l _book