跳跃运动对opgranklrank骨代谢信号通路影响的实验研究-experimental study on the effect of jumping exercise on opgranklrank bone metabolism signal pathway.docxVIP

跳跃运动对 跳跃运动对 OPG/RANKL/RANK 骨代谢信号通路影响的实验研究 英文摘要 PAGE PAGE III IV IV The Experimental Research of The Influnence of Jumping Exercise on OPG/RANKL/RANK Bone Metabolism Signal Abstract Research Purposes: Stress stimulation is one of the most basic stimulation duirong bone remodeling process Appropriate stress stimulus can promote bone cell growth, differentiation and maintain function, but the excessive or duration long mechanical force to bone cell can lead damage, the mechanism is bone cell can response to stress stimulus signal and convert to biological chemical signal , OPG, nuclear factor κ B receptor activation factor and its ligand (OPG/RANK/RANKL). Bone metabolism signal path is one of stress sensitive pathways. OPG/RANK/RANKLis the key to keep dynamic balance of bone absorption and bone formation. In addition, the hormone can also affect bone metabolism signal path, parathyroid hormone is the important hormone to regulation bone metabolism signal pathway. Continuous movement stress can enhance hormone level inevitably, whether stress and hormone can form stimulation on bone. Therefore, this experiment to study the law of OPG、RANKL during jumping exercise, and the change rule of parathyroid hormone on bone metabolism signal, from gene level to study bone metabolism, provided the evidence for other studies. Research Methods: This experiment is that rats start six weeks of jumping exercise in the cage with semi-automatic adjustable voltage, after exercise, killed rats and taken the blood and the tibia. HE dyed slice to observe the inner change of bone tissue, ELISA method was used for determining the content of serum PTH, Real-time PCR was used for determining the level of OPG、RANKL mRNA of tibia tissue, Analysis the change regulation of OPG, RANKL. Research Results: 1、HE dyeing section, fiber arrangement began to disorder, the number of bone lacunar increasing gradually, bone lacunar broadening gradually, bone trabecular 英文摘要 跳跃运动对 OPG/RANKL/RANK 骨代谢信号