首乌藤多糖抗氧化活性及提取纯化工艺分析-antioxidant activity and extraction and purification process analysis of polysaccharides from caulis polygoni multiflori.docxVIP

目 录 摘 要 ································ ································ ·············· 1 Ab s t r a ct 3 前 言 ································ ································ ·············· 5 实验 1 S W T P 抗氧化活性研究 ································ ················ 7 实验 2 S W T P 水提工艺研究 ································ ················· 13 实验 3 S W T P 碱提工艺研究 ································ ················· 19 实验 4 S W T P 酶提工艺研究 ································ ················· 30 实验 5 S W T P 脱色脱蛋白纯化研究 ································ ········ 43 实验 6 多糖含量测定方法的建立 ································ ··········· 51 讨论 ································ ································ ··············· 54 结论 ································ ································ ··············· 56 致谢 ································ ································ ··············· 57 参考文献 ································ ································ ········· 58 附录 1 文献综述 ································ ······························· 59 附录 2 在校期间论文论著情况 ································ ·············· 73 摘要 摘要 首乌藤 首乌藤多糖抗氧化活性及提取纯化工艺研究 PAGE PAGE 13 PAGE PAGE 10 摘 要 金水区科技攻关项目 目 的 ： 考察首乌藤多糖 ( SWTP)抗氧化活性，并优选 SWTP 提取纯化工 艺，为进一步开发 首乌藤药材提供依 据。 方 法 ：（ 1 ）体 外 测 定 SWTP 清除 F e n t o n 体系产生的羟基自由 基（ - OH ）、 -邻苯三酚自氧化体 系产生的超氧自由 基（ O 2 - ）的能力； 使用 超氧化物歧 化 酶 （ SOD ）、 谷 胱 甘 肽 过 氧 化 物 酶 （ G S H - Px ）、 丙 二 醛 （ M D A ） 测 定 试 剂 盒测定 SWTP 体 内 抗 氧 化 活 性 。 （ 2 ） 以多糖得率和含糖量为指标，优选 SWTP 水提工艺，并对多糖醇沉浓度进行考察。（ 3）在优选出最佳碱种类的基础上，以 NaOH 浓度(A)、提取温度(B)、料液比(C)、提取时间(D)为考察因素，确定 SWTP 碱提工艺的最优条件。（4）在确定适用酶为纤维素酶、料液比为 1:10、酶解 pH 为 5.0 的基础上，用正交试验的方法优选 SWTP 酶提工艺。。（5）采用大孔吸附树 脂法对 SWTP 的脱色、除蛋白工艺进行了研究，对树脂脱色和除蛋白的影响因素 ， 如树脂类型、上样浓度、流速、泄露曲线、洗脱体积进行了考察。（ 6）以无水葡 萄糖为对照品，绘制标准曲线，采用紫外分光光度计法研究 SWTP 含量测定方法。 结果：（1）9 m g / m L S W T P 对 - OH 的 清 除 率 是 5 1 . 7 % ，对 O 2 - 的清除率是 4 9 . 7 % ，并