细菌群体感应信号分子印迹聚合物的制备及性能分析-preparation and performance analysis of molecularly imprinted polymer for bacterial quorum sensing signals.docxVIP

哈尔滨工业大学理学硕士学位论文 哈尔滨工业大学理学硕士学位论文 - - II - Abstract Molecular imprinting technique (MIT) refers to the preparation that polymer has a specific selective to the specific template molecule. Molecular imprinting polymers are the core of molecular imprinting technology. It has three main features of schedule, recognition and practicality. Molecular imprinting technology demonstrates good applications in many areas, but these applications are mainly in analytical chemistry and related areas and in areas such as natural antibody modeling application is almost a blank. Quorum sensing is a molecular mechanism of microorganism controling gene expression through the changes in cell density. Blocking of disease resistance in pathogenic bacteria to signal induction is a original new method and a new therapeutic strategy. We select furanone which interferes with bacterial signal molecules as the template molecule in the experiment. Preparation of core-shell structure Fe3O4@SiO2-MIP of multifunctional molecular imprinted polymer beads by polymerization core-shell type silicon surface of the ball where core is Fe3O4 and shell is SiO2. The imprinted polymers can quickly combined with furanone, interfere with the signaling molecules to loss biological activity and decrease the signaling molecule concentration to below the threshold in the purpose of inhibiting the quorum sensing system. In this experiment, we use the hydrothermal method to prepare magnetic fluid Fe3O4, and then use the sol-gel method to modify its surface by TEOS to prepare core-shell micro ball of Fe3O4@SiO2. Select silane reagent KH570 to connect silicon ball surface with the double bond. On the basis of the double bond, we prepare of molecular imprinting polymer Fe3O4@SiO2-MIP. Characterize of the Fe3O4SiO2- MIP by scanning electron microscopy, thermal gravimetric analysis, XRD diffraction, infrared spectroscopy, vibrating sample magnetometer, and the EDS. The results show that the magnetic microspheres Fe3O4SiO2-