miRNA―769―5p上调对正常人成纤维细胞生物活性影响.docVIP

miRNA―769―5p上调对正常人成纤维细胞生物活性影响 　　[摘要]目的：探讨上调miRNA-769-5p表达对正常人成纤维细胞生物活性的影响。方法：将miRNA-769-5p mimics转染至人成纤维细胞中，CCK-8试剂盒测定细胞增殖率，荧光显微镜下观察氧化损伤荧光，流式细胞仪测定荧光值与细胞凋亡率，将通过转染miRNA-769-5p mimics上调miRNA-769-5p表达的转染组与未经处理的对照组成纤维细胞进行比较。结果：转染组miRNA-769-5p相对表达量为（1.36±0.2）显著高于对照组的（1.0±0.1），差异有统计学意义（P0.05）；转染组细胞增值率为（0.747±0.072）明显低于对照组的（1.007±0.025），差异有统计学意义（P0.05）；转染组细胞荧光值（92.067±3.092）明?@高于对照组的（21.600±8.487），差异有统计学意义（P0.05）；转染组细胞凋亡率为（49.299±5.184）%明显高于对照组的（5.977±0.939）%，差异有统计学意义（P0.05）。结论：上调miRNA-769-5p可诱导出类似紫外线光损伤效应，对正常人成纤维细胞生物活性产生影响。 　　[关键词]miRNA；成纤维细胞；紫外线；光损伤 　　[中图分类号]R329.2+8 [文献标志码]A [文章编号]1008-6455（2018）04-0109-03 　　Effect of miRNA-769-5p on Biological Activity of Normal Human Fibroblasts 　　NI Na，ZHOU Bing-rong，MA Wei-wei，LUO Dan 　　（Department of Dermatology，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，Jiangsu，China） 　　Abstract： Objective To investigate the effect of miRNA-769-5p on biological activity in normal human fibroblasts. Methods Human fibroblasts were transfected with miRNA-769-5p mimic. CCK-8 kit was performed to test proliferation rate. Cell were observed under fluorescence microscope. Flow cytometer was used to measured rate of reactive oxygen species and apoptosis. All the parameters of the mimics group were compared with the control group. Results The relative expression of miRNA-769-5p in the mimics group was （1.36±0.2）， which was significantly higher than that of the control group （1.0±0.1）， the difference was statistically significant（P0.05）. The cell proliferation rate in the mimics group （0.747±0.072） was significantly lower than that of the control group （1.007±0.025）（P0.05）. The fluorescence value of the mimics group （92.067±3.092） was significantly higher than that of the control group （21.600±8.487）（P0.05）. The apoptotic rate in the mimics group was （49.299 ± 5.184）%， which was significantly higher than that of the control group （5.977±0.939）%（P0.05）. Conclusion Up-regulation of miRNA-769-5p induce photo-damage and ha