MMP7在结石肠上皮不同病变中表达及意义.docVIP

MMP7在结石肠上皮不同病变中表达及意义 　　【摘要】 目的 探讨MMP-7在结直肠上皮病变的恶性转变中的表达及意义。方法 用免疫组化方法检测37例结直肠癌,23例结直肠腺瘤10例非肿瘤性息肉及10例正常结直肠组织手术标本的石蜡切片中MMP-7的表达。结果 37例结直肠癌切片中,25例MMP-7为阳性表达(阳性表达率67.6%);23例腺瘤切片中,15例表达阳性,8例表达为阴性,阳性表达率65.2%;10例非肿瘤性息肉中,1例阳性表达,9例为阴性,阳性率10%;10例正常结直肠上皮对照均未见表达。结论 MMP-7在结直肠上皮病变的恶性变中逐渐增强,在结直肠癌的发生发展中起重要作用。 　　【关键词】 结直肠癌;腺瘤;基质溶解素 　　 　　Expression and significance of matrilysin(matrix metalloproteinases-7) in various disorders of human colorectal epithelium. 　　 　　PAN Hai-yan,GUAN Xiao-dong,JIA Ying-bin. 　　The Fifth Affiliated Hostpital of Sun Yat-sen University Zhuhai Guangdong519000,China 　　 　　【Abstract】 Objective To study the expression and significance of matrilysin during m alignant conversion of human colorectal epithelium. Methods Surgical specimens from 37 cases of colorectal carcinomas, 23 cases of colorectal adenomas,10 cases of non-neoplastic polyps and 10 normal colorectal tissues were paraffin sections the expression of MMP-7. 　　Results 37 cases of colorectal biopsies,25 cases positive for MMP-7 expression(positive rate 67.6%);23 cases of adenoma biopsies,15 cases of positive expression,the expression was positive in 8 case,positive rat of 65.2%;10 cases of non-Neoplastic polyps,1 were positive,9 cases were negative,the postive rate of 10%;10 cases of normal colorectal epithelium showed no expression in control.Conclusion Secretion of MMP-7 was enhanced during m alignant conversion of human colorectal epithelium. It is believed that matrilysin play an important role during colorectal carcinogenesis and in colorectal carcinomas progression. 　　【Key words】 Colorectal carcinoma;Adenoma;Matrilysin(MMP-7) 　　 　　作者单位:519000中山大学附属第五医院普外三科 　　 　　MMP-7(matrix metalloproteinases-7)也称基质溶解素,是MMPs超家族中的一员。在肿瘤的发展、侵袭与转移中起着重要的作用。它能降解细胞外基质及基底膜,使肿瘤细胞穿出而形成转移。最近有研究表明,在结直肠上皮组织的恶性转变中MMP-7的表达增强,并且活性增强[1]。文章采用免疫组化方法研究结直肠上皮不同病变中MMP-7的表达,探讨MMP-7与结直肠肿瘤发生发展的关系。 　　1 材料与方法 　　1.1 标本 采用2002~2005年中山大学附属第五医院手术切除或经内镜切除的结直肠癌、腺瘤和息肉的蜡块标本,均经病理证实。结直肠癌37例,37例结直肠癌标本中,20例为直肠癌,4例为左半结肠癌,5例为右半结肠癌,1例横结肠