Toll样受体4核转录因子κB p65在小鼠溃疡性结肠炎中表达及意义.docVIP

Toll样受体4核转录因子κB p65在小鼠溃疡性结肠炎中表达及意义 　　[摘要] 目的 探索Toll样受体4（TLR4）和核转录因子-κB（NF-κB）在小鼠溃疡性结肠炎（UC）中的表达及意义，从而探讨该信号通路在UC发病机制中可能的作用。 方法 将20只雄性Balb/c小鼠随机分为正常组（N组）和模型组（M组），每组各10只。采用葡聚糖硫酸钠诱导UC模型，各组于造模后7 d取病变处结肠组织，进行组织学评分，HE染色观察大体形态、组织学评分和免疫组化染色观察NF-κB和TLR4的表达情况。 结果 TLR4和NF-κB在M组小鼠结肠黏膜表达显著高于N组（P ＜ 0.05）；且TLR4与NF-κB的表达呈正相关，Pearson相关系数r为0.816。 结论 TLR4和NF-κB的高表达提示该信号传导通路激活导致下游炎症因子的释放可能是UC发生的机制之一。 　　[关键词] 核转录因子-κB；Toll样受体4；溃疡性结肠炎 　　[中图分类号] R574.62 [文献标识码] A [文章编号] 1673-7210（2012）05（b）-0017-03 　　The significance of TLR4 and NF-κB p65 expression in the ulcerative colitis 　　WANG Qunru1 JIANG Mingde1 WU Xiaoling1 HUANG Li2 HU Xiaosong3 LIU Jing4 　　1.Department of Gastroenterology, Chengdu Military General Hospital, Sichuan Province, Chengdu 610083, China; 2.Department of Digestive Medicine, the Third Peoples Hospital of Mianyang, Sichuan Province, Mianyang 621000, China; 3.Department of Morphology, Chengdu Medical College, Sichuan Province, Chengdu 610083, China; 4.Department of General Surgery, the First Affiliated Hospital of Chengdu Medical College, Sichuan Province, Chengdu 610500, China 　　[Abstract] Objective To explore the might mechanism that through the significance of TLR4 and NF-κB p65 expression in the ulcerative colitis. Methods 20 Balb/c mice were randomly divided into normal group (group N) and UC model group (group M), with 10 mice in each group. Using the DSS to induce UC model, the colonic tissue were collected at 7 d in each group, and the expression of TLR4, NF-KB and pathological change were tested with immunohistochemistry and HE staining methods and histopathological score. Results The expression of TLR4 and NF-κB in M group was higher than that of group N (P ＜ 0.05), and the expression of TLR4 was parallel with that of NF-kB. Pearson correlation coefficient r was 0.816. Conclusion The inflammtory response following TLR4/NF-κB signaling pathway activated plays a role in the mechanism of UC. 　　[Key words] Nuclear factor κB; Toll like receptor 4; Ulcerative colitis