UCA1在膀胱癌中表达及临床意义.docVIP

UCA1在膀胱癌中表达及临床意义 　　[摘要] 目的 探讨UCA1 mRNA在膀胱移行细胞癌中的表达及其与病理分级、临床分期和复发的关系。方法 采用SYBR GreenⅡ实时荧光定量逆转录聚合酶链反应试验方法检测39例膀胱癌组织及8例膀胱正常组织中UCA1 mRNA的表达情况。结果 39例膀胱癌组织中UCA1 mRNA表达阳性39例,阳性率分别为100%;8例膀胱正常组织中UCA1 mRNA表达阳性3例,阳性率分别为37.5%;UCA1 mRNA的表达在癌组织和正常组织中的阳性率比较差异有显著性(P0.05);UCA 1mRNA的表达与病理分级、临床分期和复发关系密切(P0.05)。结论 UCA1参与了膀胱癌的发生发展过程,对膀胱癌的诊断、判断病理分级、临床分期和预后具有重要临床意义。 　　[关键词] UCA1;膀胱癌;实时荧光定量逆转录聚合酶链反应;SYBR GreenⅡ 　　[中图分类号] R737.14 [文献标识码] A [文章编号] 1673-9701(2010)04-16-03 　　 　　UCA1 Expression in Bladder Cancer and Its Clinical Significance 　　WANG BaosenXING JinchunZHENG Jiaxin 　　Department of Urology,the Affiliated Xiamen First Hospital of Fujian Medical University,Xiamen 361003,China 　　 　　[Abstract] Objective To investigate the expression of UCA1 mRNA in bladder transitional cell carcinoma and its relationship with pathological grade,clinical stage and recurrence. Methods The SYBR Green II real-time fluorescent quantitative RT-PCR was used to evaluate the UCA1 mRNA expression in 39 cases of bladder transitional cell carcinoma and 8 cases of normal tissue. Results All of 39 cases of bladder transitional cell carcinoma cases showed the positive expression of UCA1 mRNA,with the positive rate of 100%,and 3 of 8 cases of normal tissue showed the positive expression of UCA1 mRNA,with the positive rate of 37.5%. There was a significant difference in the positive rate of between the two(P0.05). There was a close relation between the pathological grade(P0.05), the clinical stage(P0.05)and recurrence(P0.05). Conclusion UCA1 is involved in the development and progression of bladder transitional cell carcinoma and it is of important clinical significance in the diagnosis of bladder transitional cell carcinoma and the judgement of pathological grade,clinical stage and prognosis. 　　[Key words] UCA1;Bladder cancer;Real time quantitative RT-PCR;SYBR Green Ⅱ 　　UCA1(urothelial carcinoma associated 1)是最近发现的一个新的膀胱癌的特异性非编码基因,本文运用SYBR GreenⅡ实时荧光定量RT-PCR的方法检测39例膀胱癌组织及9例膀胱正常组织中UCA1 mRNA,探讨其表达与膀胱癌临床分期和