α酮戊二酸盐对酿醋废水厌氧发酵产沼气影响.docVIP

α酮戊二酸盐对酿醋废水厌氧发酵产沼气影响 　　摘要酿醋废水接种活性污泥后在厌氧、37℃和120rpm条件下进行批培养,添加α-酮戊二酸盐(终浓度0.2g/L)促进甲烷菌的三羧酸循环碳代谢和胞内的镍同化作用,使同化镍含量比对照提高53.2%,这反映了以镍为中心离子的F430的合成和以F430为辅酶的甲基CoM还原酶活性得到增强,并最终从产气效率上反映出来,其添加α-酮戊二酸盐(终浓度0.2g/L)挥发性乙酸盐残留量比对照减少27.3%,而沼气总产量、TS和YP/V以及qp分别比对照提高了23.0%、11.1%、20.1%和10.7%,说明厌氧体系中添加α-酮戊二酸盐可以促进食醋废水中的乙酸底物代谢流向终产物甲烷而非仅用于菌群繁殖,α-酮戊二酸盐是通过提高菌群的甲烷合成能力来提高产气,而非仅通过促进菌群生长的群体优势。 　　关键词α-酮戊二酸盐;厌氧发酵;沼气;代谢 　　中图分类号X797文献标识码A文章编号 1007-5739(2009)15-0280-02 　　 　　Effectofα-ketoglutarateonAnaerobicBatchMethaneFermentationofVinegarBrewingWastewater 　　WANG Zhi 1,2,3XU Ying1,2,3 CHEN Xiong 1,2,3LIAO Wen-tan1,2,3 WANG Jin-hua1,2,3 *LI Dong-sheng1,2,3 　　(1 College of Bioengineering,Hubei University of Technology,Wuhan Hubei 430068; 2 Key Laboratory of Fermentation Engineering(Minister of Education); 3 Hubei Provincial Key Laboratory of Industrial Microbiology) 　　AbstractThe effect of α-ketoglutarate on anaerobic batch methane fermentation of vinegar brewing wastewater was investigated at 37℃ and 120rpm. The results showed that α-ketoglutarate addition of 0.2g/L facilitated carbon metabolism of TCA cycle and assimilatory Ni in methan-ogenesis,thus enhancing assimilatory Ni concentration that was 53.2% higher than control,which reflected the improvements of F430 biosynthesis with Ni as central metal ion,and suggested the enhancement of methylreductase with coenzyme of F430. The 27.3% lower residually volatile acetate,the 23%、11.1%、20.1% and 10.7% higher biogas,TS,YP/V and qp suggested anaerobic vinegar brewing wastewater fermentation with α-ketoglutarate addition facilitated biogas production not only by increasing microorganism amounts,but also by enhancing the ability of methanogens to biosynthesize methane. 　　Key wordsα-ketoglutarate;anaerobic fermentation;biogas;metabolism 　　 　　食醋酿造生产过程中产生大量高浓度有机废水,其有机物和悬浮物含量高[1],排放会导致受纳水体富营养化。国内处理废水的工艺主要包括厌氧水解-好氧处理方式以及活性污泥污水处理[2]。由于以乙酸为底物所形成的甲烷约占总量的60%[3],因而用酿醋废水厌氧发酵产沼气有其潜在的应用价值和环保价值。厌氧发酵产沼气过程是产甲烷菌利用底物水解和产酸阶段所生成的乙酸等简单底物合成CH4等混合气的过程[4],包括不产甲烷细菌为产甲烷细菌提供