SIM对人骨形态发生蛋白2诱导大鼠异位成骨X射线学研究.docVIP

SIM对人骨形态发生蛋白2诱导大鼠异位成骨X射线学研究 　　【摘要】 目的 在重组人骨形态发生蛋白-2(rhBMP-2)诱导异位成骨过程中，局部应用不同浓度的辛伐他汀(SIM)，探讨SIM对rhBMP-2诱导成骨的作用。方法 ①以牛松质骨为原料经过脱脂、脱蛋白等步骤制备牛骨基质明胶载体,将辛伐他汀和rhBMP-2分别与BMG载体复合;②将45只雄性SD大鼠随机分为3组。A组：高剂量实验组；B组：低剂量实验组；C组：对照组;③将复合体植入大鼠股后部肌袋中，术后15 d、30 d、60 d对实验大鼠进行大体观察、X线检测。结果 ①大体观察：各组组织块均随着时间延长而变硬，范围变大;②X线结果，各组植入部位术后15 d、30 d未见显影，于术后60 d各组可见不同密度的影像，B组和C组钙化明显，成高密度影像；A组成低密度影像。成骨区灰度值A组明显低于B组和C组。结论 高浓度辛伐他汀可明显抑制rhBMP-2的促进成骨作用；低浓度辛伐他汀的抑制作用则不明显；证实了rhBMP-2在促进成骨方面有重要作用。? 　　【关键词】 辛伐他汀；重组人骨形态发生蛋白-2；诱导；成骨；X射线放射学?? 　　 　　The radiology research of Simvastat on Ectopic Bone Formation of rat Induced by Recombinant Human Bone Morphogenetic 　　 　　WANG Yan, CHEN Guo-Dong,PANG Tian-Shu. 　　Department of Radiology,the Central Hospital of Liaohe Oil Field, Panjin 124000, Liao Ning,China 　　? 　　【Abstract】 Objective To investigate the synergistic effect of different concentration Simvastat(SIM)on the bone induction of recombinant human bone morphogenetic protein-2(rhBMP-2)through local application of SIM in the osteoinductive process of BMP.Methods ①Prepare the bone matrix galatin(BMG)carrier as the raw materiar from cattle spongy bone by the procedure of degrease, deproteinization and so on.To compound the BMG carrier with SIM、rhBMP-2 or both.②Forty-five male SD rat were divided into two experimental groups and control group at random,and BMG composite was implanted into the right thigh muscle pouch of each group. group A:the BMG carrier contain 15% weight ratio of SIM and 0.25 mgrhBMP-2 as high dose experimental group;group B:the BMG carrier contain 2.5% weight ratio of SIM and 0.25 mgrhBMP-2 as low dose experimental group; group C:the BMG carrier just contain 0.25 mgrhBMP-2 as control group(CG). ③At dl5, d30 and d60 after implantation, new bone formation was measured by generally, radiographically of the three groups.Results ①The carriers of each group become hard and extensive. The effect of wet weight:the groups of A, B, C were different, which the group C has the strongest effection of all