SIRT1选择性剪接变异体SIRT1―ΔExon8 mRNA在幼龄和老龄小鼠海马组织表达改变及意义.docVIP

SIRT1选择性剪接变异体SIRT1―ΔExon8 mRNA在幼龄和老龄小鼠海马组织表达改变及意义 　　【摘要】 目的：检测小鼠海马组织中是否存在SIRT1选择性剪接变异体SIRT1-ΔExon8及其mRNA在幼龄和老龄小鼠海马组织的表达水平。方法：选取10只50 d（幼龄）和10只12个月龄（老龄）雄性昆明（KM）小鼠，分别提取海马组织的RNA并进行反转录及PCR，通过琼脂糖凝胶电泳检测海马组织中的SIRT1-ΔExon8 mRNA；采用Real-time PCR（RT-PCR）技术检测SIRT1-ΔExon8 mRNA的表达。结果：幼龄和老龄小鼠海马组织均检测到SIRT1-ΔExon8 mRNA；与幼龄组相比，老龄组小鼠SIRT1-ΔExon8 mRNA表达增加。结论：老龄小鼠海马组织的SIRT1选择性剪接变异体SIRT1-ΔExon8 mRNA较幼龄小鼠的表达增加，因此SIRT1-ΔExon8可能参与了衰老的发生，提示通过调控SIRT1-ΔExon8的表达可能会延缓机体的衰老。 　　【关键词】 SIRT1-ΔExon8； 选择性剪接； 海马； 衰老 　　Expression Changes and Significance of SIRT1 Alternative Splicing Variants of SIRT1- Exon8 mRNA in the Hippocampus of Young and Aged Mice/GAO Rui-jun，LI Yan-li，WANG Rui，et al.//Medical Innovation of China，2016，13（17）：011-014 　　【Abstract】 Objective：To detect whether existence of SIRT1 alternatively spliced variants SIRT1-ΔExon8 and its mRNA expression level in young and aged mice hippocampus.Method：A total of 10 cases of 50 days （young）and 10 cases of 12 age （aging）Kunming （KM） male mice were selected，reverse transcription and PCR were carried by extracting RNA from the hippocampus tissue，SIRT1-ΔExon8 mRNA in hippocampus tissue were detected by agarose gel electrophoresis and SIRT1-ΔExon8 mRNA expression were detected by Real-time PCR （RT-PCR） technology.Result：Young and aging mice hippocampus tissue all detected SIRT1-ΔExon8 mRNA. Compared with the young group，SIRT1-ΔExon8 increased expression of mRNA in aging mice.Conclusion：SIRT1 alternative splicing variant of SIRT1-ΔExon8 mRNA expression in aged mice hippocampus tissue is increased than young mice，thus SIRT1-ΔExon8 may be involved in the occurrence of aging，point out regulating the expression of SIRT1-ΔExon8 may delay aging. 　　【Key words】 SIRT1-ΔExon8； Alternative splicing； Hippocampal； Aging 　　First-author’s address：Shanxi Medical University，Taiyuan 030001，China 　　doi：10.3969/j.issn.1674-4985.2016.17.003 　　沉默信息调节因子1（Silent information regulator 2 homolog 1，SIRT1）是一种NAD+依赖型的脱乙酰基酶，通过对组蛋白及多种非组蛋白进行去乙酰化修饰而发挥其功能[1-3]，越来越多的研究表明SIRT