CK19Galectin―3在甲状腺良恶性肿瘤中表达及其临床意义.docVIP

CK19Galectin―3在甲状腺良恶性肿瘤中表达及其临床意义 　　【摘要】 目的：检测细胞角蛋白-19（CK19）、半乳糖凝集素-3（Galectin-3）在甲状腺良恶性肿瘤中的表达情况，探讨其在鉴别诊断甲状腺良恶性肿瘤中的临床意义。方法：收集2012年1月-2013年12月在山西医科大学第一附属医院行手术切除并且经病理证实的80例甲状腺肿瘤组织标本，包括40例甲状腺癌手术标本、40例甲状腺瘤手术标本，采用免疫组化技术检测CK19、Galectin-3在甲状腺良恶性肿瘤中的表达情况，实验结果根据阳性细胞的百分率和染色强度进行评价，两组间样本率的比较用独立样本 字2检验（检验水准α=0.0167），应用灵敏度、特异度及诊断符合率评价CK19、Galectin-3在鉴别诊断甲状腺良恶性肿瘤中的价值。结果：CK19在甲状腺癌和甲状腺瘤中的阳性率分别为100%（40/40）、37.50%（15/40），差异具有统计学意义（P0.001），CK19在鉴别诊断甲状腺良恶性肿瘤中的评价结果显示：灵敏度为100%、特异度为62.50%、诊断符合率为81.25%；Galectin-3在甲状腺癌和甲状腺瘤中的阳性率分别为80.00%（32/40）、25.00%（10/40），差异具有统计学意义（P0.001），Galectin-3在鉴别诊断甲状腺良恶性肿瘤中的评价结果显示：灵敏度为80.00%、特异度为75.00%、诊断符合率为77.50%。结论：CK19、Galectin-3在甲状腺癌中的阳性表达明显增强，可作为鉴别诊断甲状腺良恶性肿瘤的重要辅助指标，联合检测可提高甲状腺癌的诊断率。 　　【关键词】 甲状腺瘤； 甲状腺癌； CK19； Galectin-3； 免疫组化 　　【Abstract】 Objective：To detect the expression of CK19， Galectin-3 in benign and malignant thyroid tumor，and to investigate the clinical significance in the differential diagnosis of benign and malignant thyroid tumor.Method：80 cases of thyroid tumor tissue specimens between January 2012 to December 2013 were collected from the first affiliated hospital of Shanxi Medical University.All specimens had been proved by pathology，including 40 cases of thyroid cancer and 40 cases of thyroid adenoma operation specimens.Immunohistochemical method was used to detect the expression of CK19 and Galectin-3 in 80 cases operation specimens of thyroid tumor.The experimental result were evaluated according to the percentage of positive cells and the staining intensity. Chi-square test of independent samples was used in comparison of sample rate between the two groups（test level α=0.0167）.The sensitivity， specificity and accuracy of diagnosis were used to investigate the diagnostic value of CK19 and Galectin-3 in distinguishing benign from malignant thyroid tumor.Result：The positive rate of the CK19 in the thyroid cancer and thyroid adenoma were 100%（40/40）and 37.50%（15/40）respectively，the difference had statistical significance（P0.001）.The evaluation r