UF100尿沉渣分析仪检测尿液白细胞红细胞和管型干扰因素研究.docVIP

UF100尿沉渣分析仪检测尿液白细胞红细胞和管型干扰因素研究 　　[摘要] 目的 探讨UF-100尿沉渣分析仪检测白细胞（WBC）、红细胞（RBC）和管型（CAST）的干扰因素。 方法 对本院8 950例患者的中段晨尿标本用UF-100尿沉渣分析仪进行尿沉渣分析，并与显微镜镜检结果进行比较。 结果 导致WBC、RBC、CAST检测出现假阳性的最主要因素分别为非晶形盐类、草酸钙结晶和粘液丝，分别占35.3%、40.1%、38.1%。导致WBC、RBC、CAST检测出现假阴性的首位因素分别为白细胞聚集、影红细胞和少量透明管型，分别占59.2%、34.5%、100.0%。 结论 尿沉渣分析仪检测干扰因素较多，应建立镜检复查标准和结果审核制度，以免临床误诊误治相关疾病。 　　[关键词] 尿沉渣分析仪；显微镜镜检；假阳性；假阴性 　　[中图分类号] R446.12 [文献标识码] A [文章编号] 1674-4721（2012）03（c）-0077-02 　　 　　Study on the interference factors of detection of WBC,RBC,CAST by UF-100 urinary sediment analyzer 　　LIU Weiping YIN Minggang ZHONG Huixiu 　　Laboratory Department of Zigong First Peoples Hospital in Sichuan Province, Zigong 643000, China 　　[Abstract] Objective To discuss the interference factors of detection of WBC, RBC, CAST by UF-100 urinary sediment analyzer. Methods 8 950 cases of midpiece morning urine samples were dectected by UF-100 urinary sediment analyzer and compared with manual microscopy． Results The main false positive factor of detection for WBC, RBC, CAST was ammonium salts (accounts for 35.3%), calcium oxalate crystals (accounts for 40.1%), and viscose rayon (accounts for 38.1%) respectively. The main false negative factor of detection for WBC, RBC, CAST was WBC accumulation (accounts for 59.2%), shadow RBC (accounts for 34.5%) and hyaline cast (accounts for 100.0%) respectively. Conclusion Many interference factors exist in urine for detection of WBC, RBC, CAST by UF-100 urinary sediment analyzer. Therefore, microscopy review standards and result verifying system are supposed to be established to avoid clinical misdiagnosis and mistreatment. 　　[Key words] Ｕｒｉｎａｒｙ ｓｅｄｉｍｅnt analyzer; Microscopy; False positive; False negative 　　 　　UF-100型全自动尿沉渣分析仪是采用流式细胞技术和电阻抗技术，利用细胞染色技术对细胞核和细胞膜等成分进行染色，根据尿中各类有形成分产生的散射光脉冲和荧光脉冲的强度、持续时间的长短以及电阻抗信号的大小来区分尿内红细胞（RBC）、白细胞（WBC）、管型（CAST）等有形成分。但由于尿液有形成分复杂，影响因素很多，且各检测指标的形态、大小、染色性方面存在相似之处，因此该仪器并不能严格区分尿液各类有形成分，常导致某些检测项目存在一定程度的结果误判，可出现一定的假阳性和假阴性。如果不予以及时发现和纠正，将会误导临床对疾病的诊断和治疗。为了避免临床误诊和漏诊，笔者对该仪器检测的