Cr6对空心莲子草光合生理特性影响.doc

Cr6对空心莲子草光合生理特性影响 　　摘要：为了研究Cr6+对空心莲子草（Alternanthera philoxeroides）的毒害作用，探讨Cr6+的毒害机制，为早期预报Cr6+的危害和环境监测提供理论依据。在不同浓度（0、5、10、20、40 mg/L）Cr6+的处理后，评价了空心莲子草的外伤毒害症状，测定了叶绿素含量和光合参数净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）和胞间CO2浓度（Ci）。结果显示，在Cr6+浓度为0～5 mg/L时，叶绿素含量和叶绿素a/b值略有升高，此后随着Cr6+浓度的增加，叶绿素含量呈明显下降趋势，而叶绿素a/b值下降趋势不明显；随着Cr6+浓度的增加，净光合速率、蒸腾速率和气孔导度呈显著下降趋势，胞间CO2浓度呈缓慢上升趋势。因此低浓度的Cr6+对空心莲子草影响不大，而高浓度的Cr6+会产生毒害作用，最终导致植株枯萎。 　　关键词：Cr6+； 空心莲子草（Alternanthera philoxeroides）； 叶绿素含量； 净光合速率 　　中图分类号：S451 文献标识码：A 文章编号：0439-8114（2015）21-5310-03 　　DOI：10.14088/j.cnki.issn0439-8114.2015.21.026 　　Chromium-induced Photosynthetic Physiological Parameters in Alternanthera philoxeroides 　　CHEN Quan-zhan， TANG Ning， ZHANG Bian-jiang， WANG Li-ke， YANG Ping 　　（Institute of Plant Resources and Germplasm Innovation， Nanjing Xiaozhuang University，Nanjing 211171，China） 　　Abstract： In order to provide theoretical basis for the early prediction of Cr6+ hazards and environmental monitoring， toxic effects of Cr6+ on Alternanthera philoxeroides were studied. With A. philoxeroides as experimental materials， treated with different concentrations of Cr6+ （0，5，10，20，40 mg/L）， the trauma symptom was observed， the chlorophyll content and photosynthetic parameters were determined. The results showed that the chlorophyll contents and ratio of chlorophyll a/b increased when the concentration of Cr6+ was 0～5 mg/L； The chlorophyll contents and the ratio of chlorophyll a/b reached the maximum value when the concentration of Cr6+ was 5 mg/L； The chlorophyll contents and ratio of chlorophyll a/b decreased with the increasing of Cr6+ concentration.With the increasing of Cr6+ concentration， the net photosynthetic rate， transportation rate and stomatal conductance decreased， while the intercellular CO2 concentration increased. Therefore， low concentration of Cr6+ had little impact on growth of A. philoeroides， and the higher concentration of Cr6+ could produce toxic action， causing withering in the end. 　　Key words：Cr6+；Alternanthera ph