qRTPCR法分析复发性SLE患者肠道双歧杆菌柔嫩梭菌数量变化.docVIP

qRTPCR法分析复发性SLE患者肠道双歧杆菌柔嫩梭菌数量变化 　　摘 要：目的 运用实时荧光定量聚合酶链式反应（qRT-PCR）法分析系统性红斑狼疮患者肠道菌群数量的变化，探讨双歧杆菌、柔嫩梭菌与复发性SLE活动的关系。方法 提取粪便细菌组DNA，运用qRT-PCR法分析此二菌绝对数量的变化。结果 SLE复发活动期双歧杆菌、柔嫩梭菌数量均较治疗后稳定期减少（Plt;0.05），对比正常对照组也均减少（Plt;0.05）。结论 双歧杆菌、柔嫩梭菌菌群数量与SLE活动度评分相关，肠道内此二菌数量减少可能与SLE病情的复发有关。 　　关键词：系?y性红斑狼疮;双歧杆菌;柔嫩梭菌;实时荧光定量PCR 　　中图分类号：R593.241 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.05.022 　　文章编号：1006-1959（2018）05-0065-05 　　Analysis of the Changes of Intestinal Bifidobacterium and Clostridium Tenella in Patients with Recurrent SLE by qRT-PCR 　　WU Xia1，XIAO Pan-pan1，XIE Xiao-ran1，SUN Xue-qian2，FU Bing-bing2，SUN Xiao-peng2 　　（1.Jiamusi University，Jiamusi 154007，Heilongjiang，China; 　　2.Department of Rheumatological Immunology，First Affiliated Hospital of Jiamusi University，Jiamusi 154002，Heilongjiang，China） 　　Abstract：Objective To investigate the relationship between bifidobacterium，Clostridium tenella and recurrent SLE activity in patients with systemic lupus erythematosus by real-time quantitative polymerase chain reaction（qRT-PCR）.Methods The DNA of fecal bacteria group was extracted，and the absolute quantity of the two bacteria was analyzed by qRT-PCR method.Results Bifidobacterium in the recurrent stage of SLE was found to be bifidobacterium.The number of Clostridium tenella was lower than that of stable period after treatment（Plt;0.05），compared with the normal control group，it was also decreased（Plt;0.05）.Conclusion The number of bifidobacterium and Clostridium tenella was correlated with the SLE activity score，and the decrease of these two bacteria in the intestinal tract might be related to the recurrence of SLE. 　　Key words：Systemic lupus erythematosus;Bifidobacterium;Clostridium tenella;Real-time fluorescence quantitative PCR 　　系统性红斑狼疮（systemic lupus erythematosus，SLE）是一种累及全身各组织的自身免疫炎症性疾病[1]，肠道内多种细菌与SLE相关。越来越多的证据表明[2]，机体免疫功能的正常与宿主肠道内微生物群和宿主免疫系统间的动态平衡密切相关。Yeoh N等[3]提出肠道菌群与自身免疫病存在一定的关联。2013年Kamada等[4]发现小鼠