HGFc―met传导通路在子宫内膜癌发生发展中作用及意义.docVIP

HGFc―met传导通路在子宫内膜癌发生发展中作用及意义 　　[摘要] 目的 检测HGF/c-met传导通路对Survivin、XIAP表达的影响，探讨HGF/c-met通路在子宫内膜癌发生发展中的作用机制。 方法 培养ER表达水平不同的子宫内膜癌细胞系HEC-1B（ER低表达）和Ishikawa（ER高表达），通过MTT法检测不同浓度HGF对两种细胞增殖率的影响；AnnexinV-FITC法检测不同浓度HGF对细胞凋亡率的影响。Western Blot检测Survivin及XIAP蛋白的表达水平。 结果 HGF可促进子宫内膜癌细胞的增殖，在80 ng/mL内具有剂量依赖性，在72 h内具有时间依赖性（P0.05）；当HGF浓度为40 ng/mL时，HEC-1B细胞24 h增值率达到（87.0±0.02）%，为最适诱导浓度。Survivin、XIAP mRNA及蛋白的表达随着HGF浓度的增高而上调，上调作用具有剂量效应关系。HGF在ER低表达的HEC-1B细胞中作用明显高于Ishikawa细胞，差异具有统计学意义（P0.05）。 结论 HGF/c-met传导通路的激活可促进子宫内膜细胞增殖，抑制细胞凋亡，HGF/c-met可能是调控子宫内膜癌发生发展的分子靶点。 　　[关键词] 子宫内膜癌；HGF/c-met传导通路；Survivin；XIAP 　　[中图分类号] R737.33 [文献标识码] A [文章编号] 1673-9701（2015）32-0001-07 　　[Abstract] Objective To detect the influence of HGF/c-met pathway on expression of survivin and XIAP and to discuss the action mechanism of HGF/c-met pathway in occurrence and ddevelopment of endometrial cancer. Methods Cell lines of endometrial cancer with different ER expression level were cultivated： HEC-1B（ER low expression） and Ishikawa （ER high expression）. The effects of HGF in various concentrations on cell proliferation rate of both cell lines were detected by MTT method， and effects on cell apoptosis rate were detected by AnnexinV-FITC method. The expression levels of survivin and XIAP were measured by western blot. Results HGF facilitated the proliferation of endometrial cells， which was dose-dependent within 80 ng/mL， and time-dependent within 72 h（P0.05）； When HCF=40 ng/mL， the 24 h proliferation rate of HEC-1B cells reached （87.0±0.02）%， and 40 ng/mL was the optimum inducing concentration. The mRNA and protein expressions of surviving and XIAP were up regulated by the rising HGF concentration， which had a dose-effect relationship. The effect of HGF on ER low expression HEC-1B cells was evidently higher than that on Ishikawa cells， the difference was statistically significant（P0.05）. Conclusion The activation of HGF/c-met pathway can facilitate the proliferation and inhibit the apoptosis of endometrial cells. HGF/c-