人β―防御素―2在肝细胞癌中表达及其意义.docVIP

人β―防御素―2在肝细胞癌中的表达及其意义 　　【摘要】 目的：探讨人β-防御素-2（HBD-2）在肝细胞癌（HCC）中的表达及其意义。方法：收集2011年8月-2014年 　　10月手术切除的原发性肝癌标本45例，肝癌癌旁组织30例，正常肝组织20例，采用免疫组化法检测各标本中HBD-2蛋白表达情况，采用聚合酶链反应（PCR）、实时聚合酶链反应（Real-Time PCR）检测HBD-2 mRNA表达情况，同时采用原位末端标记法（TUNEL法）测定癌组织的细胞凋亡指数。结果：HBD-2 mRNA及HBD-2蛋白在肝癌组织、癌旁组织和正常肝组织中的表达情况比较差异均有统计学意义（P0.05）。HBD-2蛋白表达阳性的肝癌组织的细胞凋亡指数高于HBD-2蛋白表达阴性的肝癌组织，比较差异有统计学意义（P0.01）。结论：HBD-2在肝细胞癌组织中低表达，HBD-2可能通过影响肝癌细胞的凋亡而起作用。 　　【关键词】 人β-防御素-2； 肝细胞癌； PCR； 细胞凋亡 　　Expression and Significance of HBD-2 in Hepatocellular Carcinoma/LI Rong-jiang，GUI Shui-qing，HUANG Zhen-hua，et al.//Medical Innovation of China，2015，12（23）：007-009 　　【Abstract】 Objective：To investigate the expression and significance of HBD-2 in hepatocellular carcinoma（HCC）.Method：45 specimens of HCC tissues，30 specimens of para-carcinoma tissues and 20 specimens of normal liver tissues in our hospital from August 2011 to October 2014 were collected.The expression situation of HBD-2 protein in the tissues was evaluated by immunohistochemistry，the expression situation of HBD-2 mRNA in the tissues was evaluated by PCR and Real-Time PCR，the cell apoptosis indexes in the tissues were detected by TUNEL methods.Result：The differences in the mean expression of HBD-2 protein and HBD-2 mRNA of hepatocellular carcinoma tissues，para-carcinoma tissues and normal liver tissues were statistically significant（P0.05）.The cell apoptosis indexes in the HCC tissues with positive expression of HBD-2 protein was higher than that in the HCC tissues with negative expression of HBD-2 protein，the difference was statistically significant（P0.01）.Conclusion：The expression of HBD-2 in HCC is low，it may play a part through the cell apoptosis of HCC. 　　【Key words】 HBD-2； Hepatocellular carcinoma； PCR； Apoptosis 　　First-author’s address：Bao’an District Central Hospital of Shenzhen City，Shenzhen 518102，China 　　doi：10.3969/j.issn.1674-4985.2015.23.003 　　β-防御素-2是由哺乳动物上皮细胞产生，主要存在宿主-环境的界面位置，是宿主抵御各种侵袭的第一道化学屏障的重要组成成分，在机体免疫防御中发挥着极其重要的作用。β-防御素-2除对真菌、革兰阴性菌、革兰阳性菌、螺旋