冷凝集试验和酶联免疫吸附试验对呼吸道肺炎支原体IgM抗体检测评价.docVIP

冷凝集试验和酶联免疫吸附试验对呼吸道肺炎支原体IgM抗体检测评价 　　[摘要] 目的 评价冷凝集试验和酶联免疫吸附试验对诊断呼吸道肺炎支原体IgM抗体感染的临床价值。 方法 收集我院呼吸内科2012年12月～2013年10月共计100份诊断为呼吸道肺炎支原体感染患者的痰液标本。比较①酶联免疫吸附试验与冷凝集试验的真阳性、假阳性、真阴性及假阴性。②酶联免疫吸附试验与冷凝集试验敏感度、特异度、准确度、阳性预测值、阴性预测值、阳性拟然比和阴性拟然比。 结果 酶联免疫吸附试验的真阳性、假阳性、真阴性及假阴性分别为95%、3%、97%、5%，冷凝集试验的真阳性、假阳性、真阴性及假阴性分别为43%、39%、61%、57%，差异有统计学意义（P0.05）。 酶联免疫吸附试验和冷凝集试验敏感度、特异度、准确度、阳性预测值、阴性预测值、阳性拟然比和阴性拟然比分别为95%、97%、96%、96.9%、95.1%、31.7%、0.05%及43%、61%、52%、52.4%、51.7%、1.1%、0.93%，差异有统计学意义（P0.05）。 结论 本研究认为酶联免疫吸附试验对呼吸道肺炎支原体IgM抗体敏感性、特异度、准确度、阳性预测值、阴性预测值、阳性拟然比均明显高于冷凝集试验。 　　[关键词] 冷凝集试验；酶联免疫吸附试验；呼吸道肺炎支原体；IgM抗体 　　[中图分类号] R446.6 [文献标识码] B [文章编号] 1673-9701（2015）04-0107-03 　　[Abstract] Objective To evaluate the clinical value of cold agglutination test and enzyme-linked immunosorbent assay in the diagnosis of respiratory tract mycoplasma pneumoniae IgM antibody infection. Methods A total of 100 sputum samples of the patients diagnosed with respiratory tract mycoplasma pneumoniae infection in the respiratory medicine department of our hospital were collected. ①The true positive，false positive，true negative and false negative rates of cold agglutination test and enzyme-linked immunosorbent assay were compared. ②The sensitivity， specificity， accuracy， positive predictive value， negative predictive value， positive likelihood ratio and negative likelihood ratio of cold agglutination test and enzyme-linked immunosorbent assay were compared. Results The true positive，false positive，true negative and false negative rates of enzyme-linked immunosorbent assay were 95%， 3%， 97% and 5% respectively and those of the cold agglutination test were 43%， 39%， 61% and 57% respectively， with statistically significant differences （P0.05）. The sensitivity， specificity， accuracy， positive predictive value， negative predictive value， positive likelihood ratio and negative likelihood ratio of enzyme-linked immunosorbent assay and cold agglutination test were 95%， 97%， 96%， 96.9%， 95.1%， 31.7%， 0.05% and 43%， 61%， 52%， 52.4%， 51.7%，