凝胶法检测新生儿溶血病免疫性抗体临床价值分析.docVIP

凝胶法检测新生儿溶血病免疫性抗体临床价值分析 　　[摘要] 目的 探析凝胶法在新生儿溶血病免疫性抗体检测中的临床价值。方法 以该院2011年6月―2014年6月所收治85例溶血病疑似新生儿进行研究，分别应用试管法及微柱凝胶法进行溶血病免疫性抗体检测，比较HDN免疫性抗体及HDN免疫性抗体特异性检测阳性率。结果 观察组直接抗人球蛋白试验阳性率为81.2%、抗体游离试验阳性率为87.1%、抗体放散试验阳性率为91.8%，符合率为91.8%；对照组对应阳性率分别为64.7%、70.6%、75.3%、75.3%，观察组阳性率显著高于对照组，对比差异有统计学意义（P0.05）。 结论 凝胶法检测新生儿溶血病免疫性抗体与试管法相比，所受影响因素更少，敏感性高，操作简单，临床应用价值高。 　　[关键词] 凝胶法；试管法；溶血病；免疫性抗体 　　[中图分类号] R59 [文献标识码] A [文章编号] 1674-0742（2015）05（b）-0170-02 　　Analysis of the Clinical Value of Gel Method in Detection of Hemolytic Disease of the Newborn of Antibody 　　WANG Shen 　　Department of Paediatrics， third internal medicine Maternal and child health hospital，Anyang，Henan Province ，455000 China 　　[Abstract] Objective To explore the clinical value of gel method in detection of hemolytic disease of the newborn immune antibody in. Methods 85 cases of hemolytic disease of newborn treated in our hospital in June 2011 - June 2014 year in were suspected to conduct the research， application of test tube method and detection of hemolytic disease of autoimmune antibodies microcolumn gel test， compared the positive rate of antibody and HDN antibody and specific detection of HDN comparison. Results In observation group， the direct coombs test positive rate was 81.2%， the antibody positive rate was 87.1%， the free test of antibody elution test positive rate was 91.8%， the coincidence rate was 91.8%； the positive rate of control group were 64.7%， 70.6%， corresponding to 75.3%， 75.3%， the observation group was significantly higher than those in control group， differences were statistical significance （P0.05）. Conclusion Compared to test tube method， gel method in detection of hemolytic disease of the newborn immune antibody with less factors affecting， high sensitivity， simple operation， high clinical application value. 　　[Key words] Gel method； Test tube method； Hemolytic disease； Immune antibody 　　新生儿溶血病为一种免疫性疾病，主要由母婴血型不合时母体血清中IgG抗体经由胎盘进入胎儿体内，致使胎儿红细胞被破坏所引发[1]。新生儿溶血症不会对母体来造成任何影响，但胎儿会出现严重贫血、心力衰竭等问题，严重时