HzAm1细胞rpL13基因的序列分析及病毒感染对其转录水平的影响3.PDFVIP

HzAm1 细胞 r pL 13 基因的序列分析及病毒感染对其转录 水平的影响 1 ,2 1 1 代文涛 ,邓 　菲 ,胡志红 ( 1. 中国科学院武汉病毒研究所 病毒学国家重点实验室和无脊椎动物病毒学联合开放实验室 ,武汉 430071 ;2 . 中国科学院研究生院,北京 100039) Sequence Analysis of R pL13 Gene f rom HzAm1 Cell s and Eff ect of HaSNPV Inf ection on the Transcription of R pL13 DA I Went ao1 ,2 ,D EN G Fei1 , HU Zhihong1 ( 1. S t ate Key L aboratory of Vi rology an d J oi ntL aboratory of I nvertebrate Vi rology , Wuhan I ns t i t ue of Vi rology , Chi nese ) A ca demy of S ciences , Wuhan 43007 1 , Chi na; 2 . Gra d uate S chool of Chi nese A ca demy of S ciences , B eij i ng 100039 , Chi na Abstract : The cDN A and geno mic DN A of ribo so mal p rot ein L 13 ( RpL 13) gene were cloned f ro m H el icove rp a z ea cell s ( HzA M 1) . Sequence analy si s indicat ed t hat t he op en reading f rame of rp L 13 wa s co mpo sed of 666bp wit hout int ron ,enco ding a p rot ein of 25 kDa . The sequence wa s u sed to con st r uct p hylo gentic t ree s wit h ot her know n sequence s of 15 animal rp L 13 s. The re sult sugge st ed it ha s similar top olo gy wit h t he cla ssical t axono my . Quantit ative R TPCR a ssay re vealed t hat t he t ran scrip tion level of rp L 13 declined in t he HzA m 1 cell s inf ect ed by H el icove rp a a rm i g era single nucleocap sid nucleopolyhedrovir u s ( Ha SN PV) . At 96 hour s po st infection , t he m RN A copie s of rp L 13 were about 8 % to t hat of healt hy cell s. Key wo