受胆固醇调节变异剪接体及其意义.docVIP

受胆固醇调节变异剪接体及其意义 　　【摘要】 目的：检测人肝癌细胞中LDLR的变异剪接体LDLR-?Exon4、LDLR-?Exon12，HMGCS1的变异剪接体HMGCS1-?Exon2和HMGCR的变异剪接体HMGCR-Exon?13是否受胆固醇的调节。方法：建立高胆固醇模型组、低胆固醇模型组及正常组，提取不同模型组的RNA并进行反转录，采用Real-time PCR（RT-PCR）技?g检测LDLR-?Exon4，LDLR-?Exon12，HMGCS1-?Exon2和HMGCR-Exon?13的mRNA的表达。结果：在高胆固醇模型组中，与正常组相比，LDLR-?Exon4、LDLR-?Exon12、HMGCS1-?Exon2及HMGCR-Exon?13的mRNA的表达量与各基因全长相比均明显升高（P0.05）；而在低胆固醇模型组中，与正常组相比，上述的变异剪接体mRNA的表达量与各基因全长相比均明显下降（P0.05）。结论：LDLR-?Exon4、LDLR-?Exon12、HMGCS1-?Exon2及HMGCR-Exon?13等变异剪接体受胆固醇的调节，并可能参与并调控胆固醇的代谢。 　　【关键词】 选择性剪接； 胆固醇； 变异剪接体 　　Cholesterol-regulated Variant Spliceosomes and Their Significance/PANG Yana，ZHAO Jinfeng，DONG Yuankun，et al.//Medical Innovation of China，2018，15（08）：018-022 　　【Abstract】 Objective：To detect whether LDLR，HMGCS1 and HMGCR alternatively spliced variants LDLR-?Exon4，LDLR-?Exon12，HMGCS1-?Exon2 and HMGCR-?Exon13 regulated by cholesterol in human hepatocellular carcinoma cells.Method：The high cholesterol model group and the low cholesterol model group were established，and compared with the normal group.RNA extraction of different model cells was reverse transcribed，and Real-time PCR（RT-PCR） technology was used to detect mRNA expression of LDLR-?Exon4， 　　LDLR-?Exon12，HMGCS1-?Exon2 and HMGCR-?Exon13.Result：Compared with the normal group，mRNA expression of LDLR-?Exon4，LDLR-?Exon12，HMGCS1-?Exon2 and HMGCR-?Exon13 were increased in the high cholesterol model group（P0.05）.However，mRNA expression of LDLR-?Exon4，LDLR-?Exon12，HMGCS1-?Exon2 and HMGCR-?Exon13 were decreased in the low cholesterol model group compared with the normal group（P0.05）.Conclusion：LDLR-?Exon4，LDLR-?Exon12，HMGCS1-?Exon2 and HMGCR-?Exon13 spliced variants are regulated by cholesterol and may participate in regulating the metabolism of cholesterol. 　　【Key words】 Alternative splicing； Cholesterol； Spliced variants 　　First-author’s address：Shanxi Medical University，Taiyuan 030001，China 　　doi：10.3969/j.issn.1674-4985.2018.08.005 　　低密度脂蛋白受体（Low density lipoprotein receptor，LDLR）是一种表达