响应面法对六味地黄方醇沉工艺优化研究.docVIP

响应面法对六味地黄方醇沉工艺优化研究 　　[摘要]优化六味地黄方醇沉工艺，获得最优的醇沉工艺，为醇沉过程苷类控制提供理论依据。采用PB试验设计，以莫诺苷、马钱苷、芍药苷为指标，考察醇沉前液密度、醇沉温度、搅拌速度、加醇浓度、静置温度、静置时间、醇含量、加醇方式、离心、醇洗10个参数对醇沉工艺的影响，得出六味地黄方浸膏苷类醇沉关键工艺参数。并采用BoxBehnken 响应面法对关键工艺参数与醇沉效果的影响进行研究与探讨，得出关键工艺参数之间的交互影响以及与指标成分的相关性方程。通过对各个指标结果建立二次回归模型，并对二次回归方程求解得到六味地黄方苷类醇沉的最优工艺参数：醇沉前浸膏密度为113 g?mL-1，加入95%乙醇醇沉至含醇量为34%，580 r?min-1搅拌15 min，静置17 h，离心得到醇沉上清液，验证实验结果与模型预测值接近。采用响应面法优化六味地黄方苷类醇沉工艺确定工艺参数的方法合理可行。 　　[关键词]六味地黄方；苷类；醇沉工艺；响应面法 　　[Abstract]The theoretical basis of the alcohol precipitation process control was provided， the alcohol precipitation was optimized and the relationship equation was got The monod glycoside， loganin and paeoniflorin were used as the evaluation indexes to determine the impact factors of alcohol precipitation techniques of Liuwei Dihuang decoction by the PlackettBurman experimental design and the levels of nonsignificant factors were identified Then， BoxBehnken response surface methodology was used to research and discuss the critical process parameters influence the effect of alcohol precipitation and draw interaction between key process parameters and the correlation equation with index components Through the establishment and solving the quadratic regression model of composite score， the optimum preparation conditions of alcohol precipitation techniques of liuwei were as follows： stirring speed was 580 r?min-1， standing time was 17 hours， alcohol concentration was 34%， the density of Liuwei Dihuang decoction was 113 The response surface methodology for optimized alcohol precipitation techniques of Liuwei Dihuang decoction is reasonable and feasible 　　[Key words]Liuwei Dihuang decoction； glycosides； alcohol precipitation techniques； BoxBehnken response surface methodology 　　中药醇沉技术是根据中药中的目标成分（如生物碱盐类、苷类等）存在水醇溶解度差异，使用适当浓度的乙醇将粘液质等杂质去除，保留目标成分的精制技术[1]，由于其操作简便，设备成本低廉，被广泛地应用在中药制剂生产中。但在实际生产中，醇沉时有效成分的含量会受到浸膏密度、加醇量、加醇速度、静置时间等多种因素的影响[28]。六味地黄方中的苷类成分有着增强免疫，延缓衰老的药效功能[9]，为了更好地保留六味地黄方醇沉液中莫诺苷、马钱苷、芍药苷，作者采用PlackettBurman实验设计对关键影响因素进行筛选，并采用BoxBehnken