番茄黄化曲叶病毒的快速分子检测-ResearchGate.PDFVIP

See discussions, stats, and aut hor profi les for t his publicat ion at : htt ps:///publicat ion/221714398 [Molecular detection of tomato yellow leaf curl virus (TYLCV)] Article  in  Hereditas (Beij ing) · March 2012 DO I: 10.3724/SP.J .1005.2012.00366 · Source: PubMed CITAT ION READS 1 93 4 authors, including: Chang-Bao Li Beij ing Academy of Agriculture and Forestry Sciences 15 PUBLICAT IONS   1,578 CITAT IONS    SEE PROFILE Some of the authors of this publication are also working on these related projects: Tomato genetics and breeding. View project All content following this page was uploaded by Chang-Bao Li on 26 November 2015. T he user has requested enhancement of the dow nloaded file. HEREDITAS (Beijing) 2012 3 , 34(3): 366―370 ISSN 0253-9772 技术与方法 DOI: 10.3724/SP.J.1005.2012.00366 番茄黄化曲叶病毒的快速分子检测 1 1 1 2 李常保 , 崔彦玲 , 张丽英 , 李传友 1. , 100097; 2. , 100101 番茄黄化曲叶病毒是当前世界范围内危害番茄生产的毁灭性病害。文章针对番茄黄化曲叶病毒全基因组 序列的特异区段自主设计了1 对特异性PCR 引物(上游引物TYLCV-F ：5′-ACGCATGCCTCTAATCCAGTGTA-3′, 下游引物TYLCV-R ：5′-CCAATAAGGCGTAAGCGTGTAGAC-3′), 依据PCR 扩增特异片段543 bp 的有无可以 快速、准确、高效、特异地检测出是否感染了TYLCV 病毒, 这项技术可以方便地应用到工厂化育苗的带毒性 检测、蔬菜大规模生产中植株发病情况的快速检测以及抗病毒育种, 从而为蔬菜安全可持续生产提供科技支撑。 番茄黄化曲叶病毒; 特异引物; 分子检测 Molecular detection of tomato yellow leaf curl virus (TYLCV) 1 1 1 2 LI Chang-Bao , CUI Yan-Ling , ZHANG Li-Ying , LI Chuan-You 1. Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 1