基于FIASCO技术芝麻SSR标记及其遗传多样性分析.docVIP

基于FIASCO技术芝麻SSR标记及其遗传多样性分析 　　摘要：采用FIASCO法构建了芝麻（Sesamum indicum L.）GATA＼AAAG＼AAAC微卫星富集文库，阳性克隆率62%，其中58%为完美型SSR。利用30个芝麻材料对其中10个扩增稳定、多态性好的SSR位点进行了多样性分析，等位基因数介于4～7之间，平均6.2个；有效等位基因数介于2.18～5.13之间，平均3.73。25个育成品种（系）遗传相似系数平均为0.740 7，遗传距离平均为0.258 9；5个地方品种间的遗传相似系数平均为0.659 3，遗传距离平均值为0.312 8，说明地方品种多样性比育成品种（系）丰富。AMOVA分析表明，品种（系）间变异大于品种（系）内变异，地方品种种内变异大于育成品种（系）。聚类分析表明，一把白与所有育成品种（系）聚为同一类。研究还鉴定出一些遗传差异较大的材料。 　　关键词：芝麻（Sesamum indicum L.）；SSR标记；FIASCO；遗传多样性 　　中图分类号：S565.3∶Q789 文献标识码：A 文章编号：0439-8114（2015）11-2586-08 　　DOI：10.14088/ki.issn0439-8114.2015.11.008 　　Development of SSR Markers and Analysis on Genetic Diversity in 　　Sesamum indicum L. Based on FIASCO 　　TAO Feia，TONG Yi-qinga，ZHAO Jiea，NAN Haoa，YANG Li-chuna，LI Jiang-haoa，GE Tai-minga，b 　　（a.Hubei Key Laboratory of Wetland Evolution and Ecological Restoration； 　　b. School of Environmental Studies， China University of Geosciences（Wuhan）， Wuhan 430074， China） 　　Abstract： In this study， 100 clones were randomly selected from the library enriched for GATA＼AAAG＼AAAC motifs using a modified FIASCO （Fast Isolation by AFLP of Sequences Containing repeats） protocol and sequenced in sesame （Sesamum indicum L.）. Sixty-two microsatellite loci， of which 58% were pure repeats， were isolated. 10 highly polymorphic microsatellite markers were obtained， and their polymorphism were assessed among 30 cultivars with 30 individuals per cultivar. The average number of alleles for these loci was 6.2 （range 4-7）. The effective number of alleles ranged from 2.18 to 5.13 with an average of 3.73. The expected heterozygosity was higher than the observed heterozygosity indicating the inbreeding effect due to breeding. These markers were also used to assess the genetic variation of 30 sesame cultivars. The results showed that the average genetic similarity coefficient and the average genetic distance of 25 breeding varieties were 0.7407 and 0.2589 respectively， and the average genetic similarity coefficient and the average genetic distance of 5 lan