基于GC TOF MS研究威灵仙总皂苷对佐剂性关节炎大鼠血清代谢谱影响.docVIP

基于GC TOF MS研究威灵仙总皂苷对佐剂性关节炎大鼠血清代谢谱影响 　　[摘要] 观察威灵仙总皂苷（TSCR）对佐剂性关节炎（AA）大鼠血清代谢谱的变化，探讨其治疗AA可能的作用机制。采用弗氏完全佐剂诱导AA大鼠模型，观察大鼠的组织病理学变化。利用气相色谱飞行时间质谱（GCTOFMS）结合主成分分析（PCA）和偏最小二乘法判别分析（PLSDA），对正常组、AA模型组和TSCR组3组之间的代谢谱进行分析，通过变量重要性投影（VIP）1结合P005筛选血清中潜在的生物标志物。与正常组相比，模型组大鼠血清中发现并鉴定出天冬氨酸、肌醇、苯乙醛等17个潜在生物标志物；与模型组相比，给予威灵仙总皂苷治疗16 d后，上述潜在生物标志物均有向正常水平回归的趋势。代谢组学分析揭示威灵仙总皂苷对AA大鼠具有一定的治疗作用，其机制可能与调节脂代谢、氨基酸代谢和能量代谢等有关。 　　[关键词] 佐剂性关节炎； 威灵仙总皂苷； 代谢组学； 气相色谱飞行时间质谱； 血清 　　Effect of total saponins of Clematidis Radix et Rhizoma on serum 　　metabolic profile in adjuvant arthritis rats based on GCTOFMS 　　LIU Qing1， WANG Yongzhong 2*， JIANG Hui2 ， HAN Yanquan2， LI Yuxin1 　　（1 School of Pharmacy， Anhui University of Chinese Medicine， Hefei 230038， China； 　　2The First Affiliated Hospital of Anhui University of Chinese Medicine， Grade 3 Laboratory of Traditional 　　Chinese Medicine Preparation， State Administration of Traditional Chinese Medicine， Hefei 230031， China） 　　[Abstract] To observe the effect of total saponins of Clematidis Radix et Rhizoma （TSCR） on serum metabolic profile changes in adjuvant arthritis（AA） rats， and explore its possible action mechanism for AA rats The AA rat models were induced by Freunds complete adjuvant（FCA）， and their histopathological changes were observed Gas chromatographytimeofflight mass spectrometry （GCTOFMS）， principal component analysis（PCA） and partial least squaresdiscriminant analysis （PLSDA） were employed to analyze the metabolic profile among normal group， AA model group and TSCR group Potential biomarkers in the serum were screened based on the variable importance projection（VIP） value1， P005 As compared with the normal group， 17 potential biomarkers such as aspartic acid， inositol and phenylacetaldehyde were found and identified in the serum of model group rats As compared with the model group， the above biomarkers were regulated nearly to a normal state after TSCR administration for 16 days Metabolomic analysis revealed that the total saponins of Clematidis Rad