基于UPLCQTOFMS技术北青龙衣褐变过程中成分动态变化分析.docVIP

基于UPLCQTOFMS技术北青龙衣褐变过程中成分动态变化分析 　　[摘要]该文分析不同褐变时期北青龙衣有效成分动态变化规律。采用UPLCQTOFMS/MS技术对13个褐变时期26批样品进行测定，差异性化合物通过Peakview 20/masterview10软件，依据精确质量数和同位素峰度比确定分子式，再通过与对照品及质谱数据库的二级谱图比对、裂解规律分析，并结合已有文献报道，确定结构式。结果不同褐变时期化学成分含量发生了明显的变化，鉴定或推断了北青龙衣中25个化合物结构，随着褐变时间的变化，13个萘醌类化合物中，具有胡桃醌相似母核的6个化合物及3个萘醌苷类化合物的含量显著下降，产生胡桃酮等4个萘醌衍生物；4个黄酮及2个酚酸类化合物含量显著下降；6个二芳基庚烷类化合物显著提高。该方法能够快速、准确鉴定北青龙衣的化学成分，并分析动态变化规律，为揭示褐变对中药材及果蔬成分的影响提供一种新的研究策略。 　　[关键词]液相色谱四极杆飞行时间质谱； 北青龙衣； 褐变； 动态变化 　　Dynamic variation of components in exocarp of Juglans mandshurica with 　　browning based on UPLCQTOF/MS 　　SUN Guodong， HUO Jinhai， XIE Rongjuan， WANG Weiming* 　　（Institute of Chinese Materia Medica， Heilongjiang Academy of Chinese Medicine Sciences， Harbin 150036， China） 　　[Abstract]To analyze the dynamic changes in components in exocarp of Juglans mandshurica at different browning periods Twentysix batches of exocarp of J mandshurica samples from thirteen browning periods were assessed by UPLCQTOFMS/MS The formula of different compounds were determined by accurate mass and isotopic abundance ratio from target screening function of Peakview 20/masterview10 software Then their structures were determined by analysis of MS/MS fragment or comparison with standard substances and references The contents of chemical components were changed significantly in different browning periods and twenty five compounds were identified or inferred Of the 13 naphthoquinone compounds， the contents of 6 compounds with similar parent nucleus as juglone and 3 naphthoquinone glycosides compounds were decreased significantly， and 4 naphthoquinone derivatives such as regiolone were produced； the contents of four flavones and two phenolic acids compounds were decreased significantly； and the contents of 6 diarylheptanoids compounds were increased significantly UPLCQTOF/MS method can be used to identify and analyze the chemical constituents from exocarp of J. mandshurica rapidly and accurately， and analyze the rules of dynamic changes， to reveal the br