基于UPLC―Q―TOFMS技术桑叶化学成分快速识别分析.docVIP

基于UPLC―Q―TOFMS技术桑叶化学成分快速识别分析 　　摘要：目的 采用超高液相色谱-四级杆-飞行时间质谱（UPLC-Q-TOF/MS）方法对桑叶的化学成分进行快速分析。方法 采用ACQUITY UPLC- Q-TOF/MS联用仪，以乙腈-水（含0.1%甲酸）为流动相梯度洗脱，流速0.3 mL/min，电喷雾离子源负离子模式采集检测，以Masslynx4.1软件分析数据。结果 根据质谱所提供的保留时间、精确相对分子质量及二级质谱裂解碎片信息并结合文献进行分析，共鉴定和推测了桑叶中19种化学成分。结论 该方法可快速、灵敏、全面地分析桑叶的化学成分，为探讨桑叶的药效物质基础提供依据。 　　关键词：桑叶；超高液相色谱-四级杆-飞行时间质谱；化学成分；黄酮 　　DOI：10.3969/j.issn.1005-5304.2018.02.016 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2018）02-0069-05 　　Fast Identification Analysis on Chemical Constituents in Mori Folium 　　by Using UPLC-Q-TOF/MS Method 　　LIU Jin-ling1，2， LI Wen-jiao1，2， WANG Ren1，2， LI Sen1，2， ZHANG Yu-tian1，2， ZHOU Jin1，2，3， HE Fu-yuan1，2，3 　　1. Pharmacy College， Hunan University of Chinese Medicine， Changsha 410208， China； 2. Laboratory of Supramolecular Mechanism and Mathematic-Physics Chracterization for Chinese Materia Medica， Changsha 410208， China； 3. Hunan Key Laboratory of Druggability and Preparation Modification for Traditional Chinese Medicine， Changsha 410208， China 　　Abstract： Obejective To conduct fast identification analysis on chemical constituents of Mori Folium UPLC-Q-TOF/MS method. Methods ACQUITY UPLC-Q-TOF/MS spectrometer was used， acetonitrile and water （containing 0.1% formic acid） as mobile phase with gradient elution. The flow rate was 0.3 mL/min， with ESI negative ion mode detection， data analysis with Masslynx4.1 software. Results According to the retention time provided by the mass spectrometry， the exact molecular weight and the secondary mass spectrometry were used to analyze the fragments by referring to literature， and 19 chemical constituents of Mori Folium were identified and deduced. Conclusion The method can analyze the chemical constituents of Mori Folium rapidly， sensitively and comprehensively， and provide the basis for the study of the pharmacological basis of Mori Folium. 　　Keywords： Mori Folium； UPLC-Q-TOF-MS； chemical constituents； flavonoids 　　桑?~为桑科植物桑Morus alba L.的干燥叶，分布于全国各地，性寒，味甘、苦，归肺、肝经，具有疏散风热、清肺润燥、清肝明目功效[1]。桑叶主要含有