基于ImageJ软件香樟千粒重测量及其应用.docVIP

基于ImageJ软件香樟千粒重测量及其应用 　　摘 要：研究香樟种子千粒重的变异水平，不仅有助于香樟的遗传改良，而且有助于香樟产业的发展。现有基于人工计数的香樟种子千粒重测量方法费时费力，而且容易出现误差，尤其不适合大样本的处理。该研究利用扫描仪获取标准图形，通过ImageJ软件自动计数，称重后得到千粒重，并与人工计数法进行了比较；利用该方法对江西境内具有地理代表性和古樟分布广泛的10个种源种子的千粒重进行了测定，分析了其种源及家系间的变异水平。结果表明：图像测定法能快速、准确对香樟种子进行计数，大大提高了千粒重测定的速度；江西局部种源香樟种子千粒重之间的变异主要来自于种源间，群体平均多样性指数为1.10，遗传多样性水平较低，且千粒重性状受地理气候因子影响较小。 　　关键词：香樟；千粒重；ImageJ软件；种源；变异 　　中图分类号 S792 文献标识码 A 文章编号 1007-7731（2018）10-0082-04 　　Application of ImageJ Analysis Software in Measuring 1000-Seed Weight of Cinnamomum 　　camphora 　　Zhong Yongda et al. 　　（The Key Laboratory of Horticultural Plant Genetic and Improvement of Jiangxi，Institute of Biological Resources，Jiangxi Academy of Sciences，Nanchang 330096，China） 　　Abstract：The variation research on camphor 1000-seed weight will both contribute to the improvement of genetic breeding and promote the development of camphor industry.However，the measurement of camphor 1000-seed weight based on artificial counting are time-consuming，laborious and prone to error，especially not suitable for large sample processing.In our research，we use the scanner to obtain the standard graphics，counting automatically by ImageJ software，calculating 1000-seed wright after weighing，and compared it with the artificial counting method.Then the camphor 1000-seed weight from Jiangxi provenance and its variation were analyzed.The results showed that scanner with ImageJ software provides a rapid and accurate way to the camphor seeds counting.1000-seed weight variations among camphor trees from Jiangxi mainly come from the provenance，the average diversity index is 1.10 which suggested small genetic variation level among populations，and less affected by climatic factors. 　　Key words：Cinnamomum camphora；1000-seed weight；ImageJ analysis software；Provenance；Variation 　　植物?N子形态是植物生活史循环中的核心特征之一[1]，深刻影响着植物的种子寿命、种子发芽率、实生苗建成、物种更新能力和种群分布格局等各个方面[2-6]。种子千粒重是林木分类学上的一种重要性状，在林木遗传研究上具有重要价值[7]，更是育苗中确定播种量的直接依据之一。传统的千粒重测定方法是人工计数后天平称重，经过换算获得千粒重，但人工计数费时费力，不但效率低而且误差较大。种子自动数粒仪的出现一定