复方守宫散对Lewis肺癌小鼠JAK2STAT3信号通路影响.docVIP

复方守宫散对Lewis肺癌小鼠JAK2STAT3信号通路影响 　　[摘要] 目的 观察复方守宫散对Lewis肺癌小鼠JAK2-STAT3信号通路的影响。 方法 将55只C57BL/6J小鼠按常规方法接种Lewis肺癌瘤株，6d后选取荷瘤成功的小鼠50只随机分为5组：阳性对照组、西药组、中药高剂量组、中药中剂量组、中药低剂量组，每组10只。阳性对照组：灌服生理盐水0.2mL，1次/d；中药组灌服复方守宫散高剂量组0.6mL（相当于120mg/kg），中剂量组0.4mL（相当于60mg/kg），低剂量组0.2mL，（相当于40g/kg），1次/d；顺铂组按1mg/kg给药，0.1mL/只腹腔注射。每天1次，共14d。在光镜下用HE染色法观察肿瘤组织病理形态学改变情况，用Western Blot法检测JAK2、STAT3蛋白的表达水平。 结果 各给药组JAK2、STAT3蛋白的表达水平出现不同程度降低，且JAK2降低较为明显。 结论 复方守宫散能够抑制肿瘤生长，降低 Lewis瘤细胞中JAK2、STAT3蛋白的表达，并通过此方式阻断JAK2-STAT3信号转导 。 　　[关键词] 肺癌；信号通路；实验研究 　　[中图分类号] R734.2#8195;#8195;#8195;[文献标识码] A#8195;#8195;#8195;[文章编号] 2095-0616（2014）09-17-05 　　 　　Study on the effects of Compound Shougong Powder on the JAK2-STAT signaling pathway of mice with Lewis lung carcinoma 　　SHEN#8195;Di1#8195;#8195;LI#8195;Chonghui2#8195;#8195;ZHOU#8195;Jinhua2#8195;#8195;XU#8195;Haihui1#8195;#8195;ZHOU#8195;Pengfei1 　　1.Anhui University of Chinese Medicine, Hefei 230000, China; 2.Department of Oncology, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230000, China 　　[Abstract] Objective To observe the effects of Compound Shougong Powder on the JACK2-STAT3 signaling pathway of mice with Lewis lung carcinoma. Methods 55 C57BL/6J mice were inoculated with tumor cell lines of Lewis lung carcinoma via a conventional method. 50 tumor-bearing mice were selected after six days and assigned to five groups: a positive control group, a western medicine group, a TCM group with high dosages, a TCM group with medium dosages, and a TCM group with low dosages, with 10 mice in each group. The positive control group was orally given normal saline of 0.2 mL once a day; the TCM groups were orally given compound shougong powder of 0.6mL for the group with high dosages (equal to 120 mg/kg), 0.4mL for the group of medium dosages (equal to 60mg/kg), and 0.2 mL for the group with low dosages (equal to 40 mg/kg) once a day; the Western medicine group was intraperitoneally injected with cisplatin of 1 mg/kg, 0.1mL each, once a day fo