复方中药组分对糖尿病大鼠血糖果糖胺及胰岛素水平影响.docVIP

复方中药组分对糖尿病大鼠血糖果糖胺及胰岛素水平影响 　　摘要：目的 观察复方中药组分（黄连生物碱40%、三七总皂苷35%、麦冬多糖25%）对糖尿病大鼠血糖、果糖胺及胰岛素水平的影响，并探讨其作用机制。方法 随机抽取10只大鼠作为空白组，其余大鼠采用链脲佐菌素腹腔注射制作大鼠糖尿病模型。成模大鼠随机分为中药高、中、低剂量组和二甲双胍组、模型组，每组10只。各给药组每日给予相应药物灌胃，连续4周。断头采血及尾静脉采血，葡萄糖氧化酶法检测空腹血糖（FBG），放射免疫双抗法检测胰岛素水平，果糖胺法检测果糖胺含量。结果 与空白组比较，4周后模型组FBG含量和果糖胺含量明显升高、胰岛素含量明显降低（P0.01）；与模型组比较，给药4周后中药中、高剂量组和二甲双胍组FBG和果糖胺含量降低、胰岛素水平升高（P0.05）。结论 复方中药组分可能通过降低糖尿病大鼠FBG、果糖胺含量，提高其基础胰岛素水平。 　　关键词：中药组分；糖尿病；空腹血糖；果糖胺；基础胰岛素；大鼠 　　DOI：10.3969/j.issn.1005-5304.2015.08.018 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2015）08-0067-03 　　Effects of Chinese Herbal Compounds on Levels of Blood Glucose， Froctosamine and Insulin of Diabetic Rats XU Jian-mei1， SONG Qian2， JIN Bing2， SUN Qi-wei2， GAO Pu2 （1.Beijing University of Chinese Medicine， Beijing 100029， China；2.Xiyuan Hospital of China Academy of Chinese Medicine Sciences， Beijing 100091， China） 　　Abstracts：Objective To observe the effects of Chinese herbal compounds （alkaloid of Coptidis Rhizoma 40%， total saponin of panax notoginseng 35%， and polysaccharide of Ophiopogonis Radix 25%） on levels of blood glucose， fructosamine， and insulin of diabetic rats， and explore mechanism of action. Methods Ten rats were randomly chosen as blank control group， and other rats were used to establish diabetic rat models by STZ intraperitoneal injection. Successfully molding rats were randomly divided into Chinese herbal compounds high， medium， low dose groups， metformin group， and model group， 10 rats in each group. Each rat received gavage for continuous 4 weeks. Then rat blood was collected by cutting head and taking caudal vein. FBG was detected by glucose oxidase method；insulin level was detected by radio-immunity double antibody method；fructosamine content was detected by fructosamine method. Results Compared with the blank control group， model group the levels of FBG， froctosamine content increased， while fasting insulin level were lower after 4 weeks’medication. Compared with the model group， the levels of FBG， froctosamine c