复方转移因子激发CD4T淋巴细胞实验研究.docVIP

复方转移因子激发CD4T淋巴细胞实验研究 　　【摘要】 目的用化疗药物建立免疫细胞抑制的动物模型，以免疫细胞为观察指标评价复方转移因子的提高免疫细胞的能力。方法 用环磷酰胺（CTX）灌胃（100 mg/kg）,隔天一次，连续五次，造成免疫功能抑制的动物模型，通过不同实验组用药前后免疫细胞的变化，以评价复方转移因子高、中、低（相当人用量的2、5、10 倍）剂量组是否能提高实验动物的免疫细胞。结果 复方转移因子能显著提高实验小鼠白细胞数量，复方转移因子低、中、高剂量组与模型组比较，P+T淋巴细胞的绝对数，P+T淋巴细胞的数量，并与剂量有关。? 　　【关键词】 复方转移因子；CD4+T淋巴细胞；环磷酰胺；动物模型?? 　　 　　Study on traditional medicine compound transfer factor provocate CD4+ T lymphocyte of experiment 　　 　　LI Yu-hu,MIAO Zhu-lei,CHENG Guo-qiang,et al． Shanghai University of Traditional Chinese Medicine． Shanghai 201203,China? 　　 　　【Abstract】 Objective Animal model of immunosuppression has been found by chemotherapeutics. Ability of traditional Chinese medicine compound transfer factor improve immunocyte, this result may be evaluated by immunocyte as a index.Methods Animal model of immunosuppression has been found by using cyclophosvnamide for intragastric administration, one time each other day, lavaging 5 times consecutively. Evaluated traditional Chinese medicine compound transfer factor high、medium、low dosage groups(equal to 2、5、10times of people using) improve or not immunocyte of experimental animals through changes of immunocyte before and after different experimental groups have been given to medicines. Result Traditional Chinese medicine compound transfer factor can increase remarkably amounts of WBC. Traditional Chinese medicine compound transfer factor high、medium、low dosage groups compare to model group ,P+ T lymphocyte of experimental mouses, P+ T lymphocyte of experimental mouses, concerned with its dosages. ? 　　【Key words】Compound transfer factor;WBC/ CD4+ T lymphocyte; Cyclophosvnamide; Animal model 　　? 　　HIV病毒是一种杀细胞性病毒，此病毒主要在CD4+T淋巴细胞内大量增殖并破坏细胞。而CD4+T淋巴细胞是人体中极其重要的免疫细胞，它的破坏，逐渐导致免疫功能衰竭。这样，即使对正常人来说是一个微不足道的感染，如小伤口或普通感冒,也可能致艾滋病患者于死地。因此，通常患者不是死于HIV感染本身,而是各种继发的机会性感染和肿瘤。? 　　关于HIV发病机理的研究近年来出现了一些新的观点,如“HIV病的肠道主要病灶论”认为肠免疫组织是最易感的解剖部位,在正常情况下,肠道免疫系统处于生理性活化状态和生理性低反应性状态,其生理性活化状态为HIV感染提供了大量的易感细胞。而在HIV感染时,由于危险信号的释放,调节性T细胞优先被删除等原因导致生理性低反应性状态被打破,而出现免疫