MicroRNA-34a对尿路上皮癌细胞株5637细胞迁移、侵袭和-海南医学.PDFVIP

Hainan Med J, Nov. 2013, Vol. 24, No. 22 海南医学2013年11月第24卷第22期 ·论 著· MicroRNA-34a对尿路上皮癌细胞株5637 细胞迁移、侵袭和增殖的影响 1 2 张 颖 ，张 超 (1.深圳市龙华新区人民医院手术室，广东 深圳 518100； 2.天津市肿瘤医院泌尿肿瘤科，天津 300060) 【摘要】 目的 研究microRNA-34a (miR-34a)对膀胱肿瘤细胞株5637细胞迁移、侵袭和增殖的影响。方法 在 5637 细胞株中过表达miR-34a，运用小室迁移实验和小室侵袭实验检测细胞迁移力和侵袭力；运用血球计数板法和新 型四唑氮盐(MTS)法检测细胞增殖力。结果 过表达miR-34a后，5637 细胞迁移力和侵袭力均下降(P0.01) ，细胞增 殖受抑(P0.0 1)。结论 过表达miR-34 能抑制膀胱肿瘤细胞株5637 的迁移、侵袭和增殖。 【关键词】 尿路上皮癌；迁移；侵袭；增殖；microRNA-34a 【中图分类号】 R737.15 【文献标识码】A 【文章编号】 1003—6350(2013)22—3277—04 EffectsofMicroRNA-34aonthecellmigration,invasionandproliferationofbladdertumorcellline5637. 1 2 ZHANGYing ,ZHANGChao .1.OperatingRoom,PeoplesHospitalofLonghuaNewDistrictofShenzhen,Shenzhen 518100,Guangdong,CHINA;2.DepartmentofUrologyOncology,TianjinCancerHospital,Tianjin300060,CHINA 【Abstract】 Objective To evaluate the effects of microRNA-34a (miRNA-34a) on cell migration, invasion and proliferation of bladder cancer cell line 5637. Methods miR-34a was overexpressed in bladder cancer cell line 5637. Transwell migration assay and transwell invasion assay were performed to predict cell migration ability and in- vasion ability, respectively. Cell proliferation was assessed by cell counting assay and MTS assay. Results The over- expression of MiR-34a significantly down-regulated cell migration and cell invasion of bladder cancer cell line 5637 (P0.01), and significantly decrease