STIM1在下咽癌中的表达及其作用机制的研究-耳鼻咽喉科学专业论文.docxVIP

万方数据 万方数据 目 录 中文摘要···························································································· （1） 英文摘要···························································································· （2） 论文题目···························································································· （3） 材料与方法························································································· （3） 结果···································································································（8） 讨论···································································································（12） 结论···································································································（14） 参考文献···························································································· （14） 文献综述···························································································· （17） 参考文献···························································································· （27） 攻读学位期间发表文章情况···································································· （31） 个人简历···························································································· （32） 致谢···································································································（33） 1内蒙古医科大学硕士研究生学位论 1 内蒙古医科大学硕士研究生学位论文（2015） 中文摘要 目的：钙离子输入（SOCE）作为维持细胞内 Ca2+平衡的重要途径在细胞内发挥着 重要的生物学作用。STIM1 是 SOCE 的重要组成部分，在细胞内起到 Ca2+感受器和 SOCE 激发器的双重作用。研究表明 STIM1 与肿瘤的发生发展关系密切，但它在下咽 癌中的功能还尚未知晓。本文通过慢病毒感染沉默人下咽癌细胞系 Fadu 细胞中的 STIM1 基因，来深入研究 STIM1 对下咽癌的影响。方法：利用 Real time PCR 和免疫 组织化学法分别检测下咽癌细胞和组织中 STIM1 的表达水平。对处于生长对数期的 Fadu 细胞进行慢病毒感染，STIM1-siRNA 沉默的 Fadu 细胞作为实验组；阴性慢病毒 对照感染的 Fadu 细胞作为对照组。利用 Real time PCR 和 Western blot 检测慢病毒感染 效率。利用 BrdU 实验、 FACS 法、PI-FACS 法 、克隆方法、 Cellomics 法分别检测 STIM1 对 Fadu 细胞的增殖、凋亡、周期克隆能力、增殖活性等方面的影响。结果： 1.STIM1 在下咽癌细胞和组织中均显著高表达，p0.05；2.慢病毒成功感染 Fadu 并使 STIM1 沉默，p0.05；3.STIM1 沉默后，Fadu 细胞的增殖受到抑制、凋亡率明显增 加、G1 期下的细胞数量明显增多、G2/M 期下的细胞数量明显减少、克隆能力减弱并 且其增殖活性受