WRAP53与食管鳞状细胞癌临床病理特征及预后关系的分析-心胸外科专业论文.docxVIP

汕头大 汕头大学医学院硕士学位论文 II II (P=0.026)，与年龄、性别、病理分化程度无明显相关性（P＞0.05）。 3、WRAP53 阳性表达的食管鳞状细胞癌患者 3 年生存率为 35.1％(13/37)，中位生存时间 为 29.9 个月，WRAP53 阴性表达的患者生存率 84.6％(11/13)，中位生存时间为 37.4 个 月。经 Kaplan-Meier 法分析，两组总生存期差异有显著统计学意义(P=0.006)。 结论: 1. 初步证明 WRAP53 蛋白在食管鳞状细胞癌组织中的表达较癌周正常粘膜上皮组织中的 表达上调。 2. 食管鳞状细胞癌组织中 WRAP53 蛋白主要集中分布于细胞核，与细胞增殖相关性； WRAP53 蛋 白 表 达 与 食管 癌 原 发 肿 瘤 浸 润 程度 、 临 床 分 期 显 著 正相 关 (P=0.001、 P=0.007)，与区域淋巴结转移正相关(P = 0.026)，与年龄、性别、病理分化程度无 明显相关（P0.0 5）。WRAP53 表达升高可以作为判断食管鳞状细胞癌侵蚀、转移的 生物学指标。 3. WRAP53 蛋白阴性表达患者预后明显优于阳性者，WRAP53 过表达是食管鳞状细胞癌患 者预后不良的生物学指标。 关键词: WRAP53； 食管鳞状细胞癌；免疫组化 PAGE PAGE IV Abstract Objective The underlying molecular mechanism of the development of Esophageal squamous cell carcinoma (ESCC) is still poorly understood, the newly discovered WRAP53 (also denoted TCAB1 or WDR79) has been noticed as a natural antisense transcript to tumor suppressor gene p53,which overexpression had been confirmed in a variety of different organizations caner cells,but WRAP53 protein expression and its functional role in ESCC are unknown.The aim of this study is to clarify the expression feature of WRAP53 protein,and correlation between WRAP53 expression and clinicopathological characters and prognosis in ESCC. Methods Alteration of WRAP53 protein was analyzed by Western blotting in 45 paired esophageal squamous cell carcinoma and nonneoplastic esophageal mucosa tissues.Immunohistochemistry (IHC) was used to evaluate expression of WRAP53 protein in 115 ESCC tissues and 85 nonneoplastic esophageal epithelium tissues (including the former 45 pairs of tissues) and correlate WRAP53 expression with clinicopathological parameters of ESCC patients.The 50 cases were followed up to December 31, 2012.The clinical material Excel database were retrospectively analyzed by Kaplan-Meier,and analyze the correlation of WRAP53 with prognosis of ESCC. Results WRAP53 protein levels were upregulated in ESCC tissue compared with corresponding nonneoplastic esophageal epithelium (P＜0.0