活性导向分离瓜蒌皮中具有抗血管紧张素转化酶作用成分お.docVIP

活性导向分离瓜蒌皮中具有抗血管紧张素转化酶作用成分お 　　[摘要]该试验基于活性导向分离瓜蒌皮中具有抗血管紧张素转化酶（ACE）作用的成分。瓜蒌皮经水提醇沉后取上清液，然后透析截留获1 000以上的部分得GLP，GLP经活性跟踪测定，DEAE Sepharose Fast Flow阴离子交换树脂分离和Superdex75系列凝胶纯化后得GLP11，并对GLP11进行HPGPC分析、单糖组成测定和ACE抑制活性检测。结果显示，采用活性跟踪方法，从瓜蒌皮中得到一种具有ACE抑制作用的均一低聚糖GLP11，相对分子质量为1 367，主要由阿拉伯糖、甘露糖和葡萄糖组成，3种单糖的摩尔比为02∶43∶100，GLP11表现出较强的ACE抑制活性，其IC50为（1134±86） mg?L-1。从瓜蒌皮中成功分离到具有ACE抑制作用的低聚糖，该发现为瓜蒌皮的化学物质基础提供依据。 　　[关键词]瓜蒌皮； 低聚糖； 活性导向分离； 血管紧张素转化酶（ACE）抑制作用 　　Bioactivityguided isolation of antiangiotensin converting 　　enzyme constituents from Trichosanthis Pericarpium 　　WANG Huijun， KE Ying， YE Guan* 　　（Shanghai Pharmaceuticals Holding Co.， Ltd.， Shanghai 201203， China） 　　[Abstract]To isolate the antiangiotensin converting enzyme （ACE） constituents from Trichosanthis Pericarpium based on bioactivityguided separation Trichosanthis Pericarpium was extracted with boiling water and precipitated by ethanol， then its supernatant was collected and dialyzed The retentate of the fractions above 1 000 was lyophilized to obtain GLP， which was then successively separated by DEAE Sepharose fast flow anionexchange and Superdex75 gel permeation chromatographic steps to achieve GLP11 A combination of HPGPC， monosaccharide compositions determination and ACE inhibitory activity studies was performed to investigate the structure and bioactivity The results showed that an antiangiotensin converting enzyme oligosaccharide， GLP11， was obtained from Trichosanthis Pericarpium based on activity tracking， whose average molecular weight was estimated to 1 367； mainly composed of arabinose， mannose， and glucose at a ratio of 02∶43∶100 GLP11 showed potent antiangiotensin converting enzyme effect with the IC50of （1134±86） mg?L-1 In this study， an oligosaccharide with antiangiotensin converting enzyme effect was isolated from Trichosanthis Pericarpium， which could lay the foundation for the substance basis study of Trichosanthis Pericarpium. 　　[Key words]Trichosanthis Pericarpium； oligosaccharide； activityguided isol