不同喂养方式婴儿肠道菌群分布特征的初步研究-病原生物学专业论文.docxVIP

万方数据 万方数据 Preliminary study of intestinal flora distribution in infants by different feeding patterns Abstract Objective：To investigate the characteristic of intestinal flora in healthy infants by different feeding patterns with the method of bifidobacteria counting and the analysis of ERIC-PCR fingerprinting. Methods：62 cases of healthy infants (30~120 days) from the Child Health Clinic of different hospitals in Qingdao were involved in the study. They were divided into four groups according to their feeding patterns which were breast, imported powdered milk, domestic powdered milk and mixed feeding. The samples of their fresh feces were collected. Bifidobacteria were isolated in anaerobic box and their colonies were counted in one part of each sample. For another part, total DNA of intestinal flora was extracted and ERIC fingerprints were amplified with the method of ERIC-PCR (enterobacterial repetitive intergenic consensus-polymerase chain reaction). After that, two specific bands (A: 1100bp, B: 1000bp) observed in different groups were cloned and sequenced. Homologous sequence and the function of their encoding proteins were analyzed by Blast ( HYPERLINK /Blast.cgi Basic Local Alignment Search Tool). Results：The colonies of bifidobacteria were more in breast and mixed feeding groups (9.101±1.33cfu/g;8.62±1.35cfu/g) than that of domestic and imported powdered milk feeding groups (7.62±1.22cfu/g;7.32±0.8cfu/g, P0.05); there was no significant difference both between breast and mixed feeding groups, and two powdered milk groups. The bands of ERIC fingerprints of all individuals’ were abundant. There were two specific bands found (A: 1100bp, mainly found in breast, domestic powdered milk and mixed feeding groups; B:1000bp, mainly found in imported powdered milk feeding group). Results of sequencing proved that two different fragments were obtained from each band (A1/A2;B1/B2). Analysis of Blast showed that all four sequences were traced to bifidobacterium longum. Th