川芎提取液对小白鼠离体子宫平滑肌收缩性能影响.docVIP

川芎提取液对小白鼠离体子宫平滑肌收缩性能影响 　　[摘要] 目的：观察不同浓度的川芎提取液对小白鼠离体子宫平滑肌收缩性能的影响。方法：30只小白鼠随机分为3组：高浓度组、中浓度组和低浓度组，每组10只；实验前3 d，每天皮下注射己烯雌酚0.2 ml/只。实验时颈椎脱臼处死小白鼠，剖宫取出子宫，置于与压力换能器相连的麦氏浴槽中，持续供氧，记录正常收缩曲线10 min，然后加入不同浓度的川芎提取液，分别记录加入川芎提取液后子宫的活动曲线。结果：川芎提取液对小白鼠离体子宫平滑肌具有抑制作用，浓度越大，抑制作用越明显（P＜0.05）。结论：川芎提取液对子宫平滑肌具有明显的抑制作用。 　　[关键词] 收缩性能；离体子宫；川芎提取液；小白鼠 　　[中图分类号] R285.5[文献标识码]A [文章编号]1673-7210（2009）06（b）-019-02 　　 　　Influence of Chuanxiong Extract on the contraction performance of in vitro mice uterus smooth muscle 　　NING Qijuan1#, HUANG Jinhua1#, DENG Shifu1#, ZHAO Shanmin2*, HE Xianjiao2 　　(1.Youjiang Medical College for Nationalities, Baise533000, China; 2.Department of Physiology, Youjiang Medical College for Nationalities, Baise533000, China) 　　[Abstract] Objective: To observe Chuanxiong Extract on the contraction performance of in vitro mice uterus smooth muscle. Methods: 30 mice were randomly divided into 3 groups: high concentration group, moderate concentration group and low concentration group, with 10 mice in each group. 3 days before the experiment, 0.2 ml Stilbestrol were given to each group every day via subcutaneous injection. Then the mice were killed through cervical dislocation, and the uterus was taken out and placed in culture medium, then put into pressure transducer connected with Michaels bath, continued supplying oxygen. The normal curve of the uterine contraction was recorded for 10 min, then different concentrations of Chuanxiong Extract were added, the activity curves were recorded separately after adding Chuanxiong Extract. Results: Chuanxiong Extract had an inhibitory effect on in vitro uterine smooth muscle; with the increase of concentration, the inhibitory effect was more obviously (P0.05). Conclusion: Chuanxiong Extract has a significantly inhibitory effect on uterine smooth muscle. 　　[Key words] Contraction performance; In vitro uterus; Chuanxiong Extract; Mice 　　 　　川芎为伞形多年生草本植物，性辛，温，无毒，入肝、胆、心包经，含挥发油和内酯化合物如川芎内酯、川芎酚，生物碱如川芎嗪等，其生物总碱及川芎嗪具有显著的强心作用，使心肌收缩力加强，心率加快，