应用微卫星标记对两个SD大鼠封闭群遗传学研究.docVIP

应用微卫星标记对两个SD大鼠封闭群遗传学研究 　　摘要：利用7个微卫星标记结合高分辨率全自动核酸分析系统，对四川和上海两个不同地区生产的SD大鼠封闭群进行遗传检测，计算并分析其群体遗传学参数。结果在两个SD大鼠群体中共发现等位基因40个，每位点等位基因数4～8个，平均5.714 3个；平均期望杂合度为0.706 8；平均多态信息含量为0.663 4。两个群体分别检测到38和36个等位基因；平均期望杂合度分别为0.706 9和0.697 9；平均多态信息含量分别为0.655 1和0.646 1；两个群体分别有4个位点和5个位点符合Hardy-Weinberg平衡。两群体间Nei（1972）遗传同一性和遗传距离分别为0.268 2和0.764 8，Nei（1978）无偏遗传同一性和遗传距离分别为0.238 1和0.788 2，不同微卫星位点的平均Fst值为0.090 5，表明两个种群的遗传分化程度为中等分化。试验结果表明，两个种群都符合封闭群动物的群体遗传特征，是比较理想的封闭群。 　　关键词：SD大鼠；封闭群；微卫星；遗传多样性 　　中图分类号：Q75 文献标识码：A 文章编号：0439-8114（2017）17-3308-04 　　DOI：10.14088/ki.issn0439-8114.2017.17.030 　　Genetic Analysis on Two Populations of Closed Colony Sprague-Dawley Rats Using Microsatellite DNA Markers 　　HOU Kai-ling， ZHANG Lei， ZHOU Jing， XIA Jie-ying 　　（Sichuan Academy of Chinese Medicine Sciences， Chengdu 610041， Sichuan， China） 　　Abstract： 7 microsatellite markers were screened by a full-automated high-resolution nucleic acid analysis system for the genetic analysis on two closed populations of Sprague-Dawley rats from two districts of Sichuan and Chengdu， and the population genetic parameters were calculated. The results showed that a total of 40 alleles were detected in two populations， with 4-8 alleles and a mean 5.714 3 alleles at each locus. The mean expected heterozygosity was 0.706 8，and the mean polymorphism information content was 0.663 4. In the two populations，38 and 36 alleles were detected，the mean expected heterozygosity was 0.706 9 and 0.697 9，and mean polymorphism information content was 0.655 1 and 0.646 1，respectively. Four loci and five loci， respectively，were in accord with Hardy-Weinberg equilibrium in the two populations. The Nei（1972） genetic identity and genetic distance between the two populations were 0.268 2 and 0.764 8，respectively. The Nei（1978） unbiased genetic identity and genetic distance between the two populations were 0.238 1 and 0.788 2，respectively. The average Fst of all loci was 0.090 5，which implied a moderate genetic differentiation between two popul