猪组织工程皮肤构建及移植的实验分析-experimental analysis of pig tissue engineering skin construction and transplantation.docxVIP

第三军医大学硕士研究生论文 第三军医大学硕士研究生论文 PAGE PAGE 1 英文缩写与注释 缩写 ABC 英文全称 avidin-biotin complex 中文译名 卵白素生物素复合物 BM CF basement membrane chemotactic factor 基底膜 趋化因子 DAB Diaminobezidin 二氨基联苯胺 DE Dermal equivalent 真皮替代物 DMEM Dulbecco s modified Eagle’ s Medium DMEM 培养基 DMSO dimathyl sulfoxide 二甲基亚砜 ECM EDTA extra-cellular matrix ethylenediamine tetra acetic acid 细胞外基质 乙二胺四乙酸 EGF epidermal growth factor 表皮生长因子 FB fibroblast 成纤维细胞 FCS fetal calf serum 胎牛血清 FGF fibroblast growth factor 成纤维细胞生长因子 FN GAG Fibronectin Glycosaminoglycan 纤维连接蛋白 糖胺聚糖 HE hematoxylin eosin 苏木素伊红染色 HA hyaluronan 透明质酸 HEPES N-2-hydroxyethlpiporazine piperazine 羟乙基哌嗪乙磺酸 ethanesulfonic acid HGF hepatocyte growth factor 肝细胞生长因子 HSE human skin equivalent 人皮肤替代物 IL interleukin 白细胞介素 IG immunoglobulin 免疫球蛋白 IGF insulin-like growth factor 胰岛素样生长因子 IGFBP insulin-like growth factor binding protein 胰岛素样生长因子结合蛋白 K KC keratin keratinocyte 角蛋白 角质形成细胞 KGF keratinocyte growth factor 角质形成细胞生长因子 KGM keratinocyte growth medium 角质形成细胞培养基 LN Laminin 层粘蛋白 NCS newborn calf serum 新生小牛血清 PBS phosphate-buffered saline 磷酸盐缓冲液 PDGF rpm platelet derived growth factor revolutions per minute 血小板源性生长因子 转/分 SE skin equivalents 皮肤替代物 SP Strepavidin Peroxdase 链霉菌抗生物素蛋白-过氧化 酶免疫组化试剂盒 TDM three-dimensional model 三维模型 TGF VEGF transforming growth factor vascular endothelial growth factor 转化生长因子 血管内皮生长因子 Experimental study on fabrication and transplantation of swine composite chitosan tissue engineered skin Abstract Objectives:To provide experimental evidence for clinical application of the composite chitosan tissue engineered skin, a swine tissue engineered skin was fabricated to study the viability of the seeded cells,change of epidermal differentiation markers,reformation of the blood vessles and basement membrance after autograft and allograft. Methods: the swine keratinocyte (KC )and fibroblast (FB )cell bank was established in vitro by cell culture techniques. Serially passaged cells marked with BrdU were used to fabricate the composite chitos