蛛网膜下腔出血后小窝蛋白-1caveolin-1与基底动脉平滑肌增殖的相关性研究-relationship between caveolin - 1 cave olin - 1 and proliferation of basilar artery smooth muscle after subarachnoid hemorrhage.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 PAGE PAGE 3 Study on the relationship between expression level of caveolin-1 and proliferation of vascular smooth muscle cells after experimental subarachnoid hemorrhage Abstract Backgroud and Purposes: As a seriuouly complication of subarachnoid hemorrhage (SAH), delayed cerebral vasospasm (DCVS) has a high incidence and is the main occasion leading a poor mobility and mortality of the SAH patients. The prevention and reversion of CVS has become a tough problem in front of our neurosurgeon and neurobiologist. The mechanism research of CVS after SAH has already been in progress for decades, but the origin is still a mystery. The past researches showed that the functional disorder of vascular endothelial cells, proliferation of vascular smooth muscle cells(VSMCs), inflammatory reaction, gene regulation and so on all take part in the mechanism. Although the conception that CVS is reduced by multifactors and multi-links has been widely accepted, the key point has not been fully recognized. Many experiments had indicated that the continuous vasospasm was related with the apoptosis of endothelial cells, reconstruction of vessel wall, constriction and proliferation of VSMCs. Especially, VSMCs may play an important role in the DCVS. Caveolae is a special micro-domain on the cell membrane, consist of caveolin, cholesterol, sphingomyelin and glycosyl sphingomyelin. Caveolae participate in the physiological processes of celluar signal transduction, endocytosis and pinocytosis, metabolism of membrane phospholipid. Caveolin-1 is rich in the membrane of the VSMCs, it combines with many adaptor molecule such as tyrosine kinase, growth factor receptors, endothelial nitric oxide synthase, G protein and G protein-coupled receptors as a negative regulator. Caveolin-1 has been found a close relationship with the phenotypic alternation of VSMCs in the research field of cardiovascular diseases. As the “main valve” of signal transduction of VSMCs, we can pred