（毕业设计论文）《利用反相高效液相色谱(RP-HPLC)分析和制备多肽》.docVIP

摘要 随着现代生物技术的发展,出现了各种多肽类药物,多肽在临床医学中有巨大的应用价值。自从发明固相法化学合成多肽以来,有关各种活性多肽的化学合成有了很大发展1] ,但产物成分比较复杂,一般是以目标多肽为主的几种结构相似的多肽混合物,因此,对其分离纯化的最优化研究就显得格外重要。此外,提供高纯度的多肽样品,对于其物化性质、生物活性及其医药功能的研究具有重要的作用。从现代分离科学理论得出,色谱和电泳是目前所知的分离效果最佳的两种方法。电泳仅能用于分离而不能用于多肽的制备[2 ~4] 。反相高效液相色谱(RPLC) 具有分离效果好、分辨率高、回收率高的特点,在多肽的分离纯化和制备中备受青睐,因此,RPLC是目前分离纯化和制备多肽的主要手段4~9] 。本文拟用对化学合成的肽进行分离和制备。 关键词：多肽；反相高效液相色谱 Abstract As the development of modern biological technology, many peptide medicines have been invented and produced. In the field of the clinical medicine, the peptide holds the massive value. Since the invention of the solid phase synthesis, chemical synthesis related a great number of activated peptide has skyrocket advancement [1]. However, the production contains certain impurities which have the similar structure as contrast the target peptide, therefore, it is important that the optimization research of the purification. And then, high purity peptide also can help the research of the physical and chemical character, biological activity and medicament function. We can conclude that the chromatography and electrophoresis are the best isolation effect methods at present.Nevertheless, the electrophoresis only use the qualitative experiments not preparation [2 ~4]. RP-HPLC holds high isolation, resolution and recycle ratio. As the upward reasons, RP-HPLC is the major method of the peptide purification in this world[4~9]. Key Words: Peptide; RP-HPLC; Separation; Preparation 目 录 1 前言与文献综述 ………………………………………………………1 1.1 HPLC简介…………………………………………………………………………1 1.1.1HPLC起源…………………………………………………………………1 1.1.2HPLC发展……………………………………………………………2 1.1.3HPLC 前景……………………………………………………………3 1.1.4HPLC 原理……………………………………………………………4 1.1.5RP-HPLC的应用与优点…………………………………………5 1.2多肽的简介……………………………………………………………6 1.2.1 多肽的合成方法……………………………………………………………6 1.2.2多肽的应用……………………………………………………………7 2 多肽的纯化实验………………………………………………………10 2.1 仪器以及用具………………………………………………………10 2.2试剂………………………………………………………………10 2.3实验方案………………………………………………………10 3 结果与讨论………………………………………………………12 3.1 粗肽的RP-HPLC